Basic HPLC and CD of Biomolecules [Review]


	
	
	




	Issues in Science and Technology Librarianship
	Spring 1999



	DOI:10.5062/F41Z42CB






Book Reviews

Basic HPLC and CE of Biomolecules



Venkat Raman

Chemical Abstracts Service

Columbus, Ohio

vraman@cas.org




Basic HPLC and CE of Biomolecules. Robert L. Cunico, Karen M. Gooding, 
and Tim Wehr. Richmond, CA: Bay Bioanalytical Laboratory, 1998. 388 pp., $39.95
(ISBN 0-9663229-0-8)



High-performance liquid chromatography (HPLC) has become
the workhorse of the biotechnology and pharmaceutical
industries where it is used to identify, characterize, and
purify molecules at all stages of a process from R&D to
quality assurance and validation. The book discusses the
fundamental aspects of HPLC as they pertain to biomolecules so
that the reader can choose and modify chromatographic methods
using appropriate instrumentation. The Introductory chapter
is followed by those on the Theory of HPLC, HPLC hardware, HPLC
detectors, HPLC packings, Size exclusion chramatography,
Reversed-phase chromatography, Hydrophobic interaction
chromatography, Ion-exchange chromatography, Affinity chromatography
and related techniques, Colum handling and maintenance,
Capillary electrophoresis, Sample preparation, Quantitation
and data handling, Troubleshooting, and Method development
and validation.

There are two Appendixes on Bibliographic Resources and a
Glossary. The book is well written, logically organized, and
includes the essential details to practice liquid chromatography
and capillary electrophoresis. It has an excellent balance between
theory and essential facts.








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