A8-428~9,000-L18O TEXAS AGRICULTURAL EXPERIMENT STATION A. B. CONNER, DIRECTOR COLLEGE STATION. BRAZOS cons“. ‘runs BULLETIN NO. 380 MAY, 1928 DIVISION OF EN TOMOLOGY INVESTIGATIONS ON CONTROL OF COTTON FLEA HOPPER IN I927 AGRICULTURAL AND MECHANICAL COLLEGE OFT TEXAS T. O. WALTON, President STATION STAFFT ADMINISTRATION: VETERINARY SCIENCE: A. B. CONNER, M. S., Director ,_*M. FRANCIS, D. V. M., Chief R. E. KAIRPER, B. S., Acting Vice-Director H. SCHMIDT, D. V. M., Veurinarian .11“ SIfIIHAEDEL, Sficretigliit f Cl k J . D. JoNEs, D. V. M., Veterinarian e . . OLLEMAN, R., ie er _ J. K. FRANCKLOW, Assistant Chief Clerk pljA§TTgégalighgGgh AgDcIhi-PIYSIOLOGY‘ CnEsTEn HIGGS, Executive Assistant L’ PESSIN Ph ‘D ‘ Plant P "I i -t dl CIJEBLEHE, Technical Assistant ' Laboratoiiy TéChhi-ci-an a 00g" an ' TRY: - ~ G. s. FRAPS, Ph. D., Chief; State Chemist ypflufififfisygsiqtp'i,'if,,fjai,'j§ifgf‘isi % ‘ESBURY’ MB SS’ Aézictant Chemist I B F DANA M Plant Pathologisat . . ARLYLE, . ., emist ' ' ’ ' " WALno H. WALKER, Assistant Chemist FARM AND RANCH ECONOMICS: . VELMA GRAHAM, Assistant Chemist , L- P- GABBARD- M- -- C c . R_ Q_ BROOKE, M_ S“ Assistant Chemist G. L. CnAvqi-"onn, M. S., Marketing Research; T. L. OGIER, Assistant Chemist Spcclallct J. G. EVANS, Assistant Chemist 6' A- B°NNEN- M: s.“ 1'71"" Manaccmcm ATiiAN J. STERGES, B. S., Assistant Chemist Research Spcclallst . . ‘ G_ $_ CRENSHAW, A_ B" Assistant Chemist “V. L. Comr, M. S., Grazing Research Botanist JEANNE M_ FUEGAS’ Assistant Chemist T. L. GASTON, Jn., B. S., Assistant; Farm HQRTICULTURE: Records and Accou_nts _ HAMILTON p_ TRAUB; ph_ D" Chief **J. N. TATE, B. S., Assistant; Ranch Recordr H. NEss, M. S., Berry Breeder and Accounts RANGELANIMAL HUSBANDRY: RURAL HOME RESEARCH: J. M. JoN_Es,_A. M., Chief; Sheep and Goat JEssiE WurrAcnE, Ph. D., Chief ’ Investigations _ MAMIE GRIMES, 1\I. S., Textile and Clothing. J. L. LUSLi, Ph. D.,_An_imal Husbandman; Specialist .~ Breeding Investigations EMMA E. SUMNER, M. S., Nuirition Specialist‘ STANLEY P. DAvis, Wool Grader SOIL SURVEY: ENTOMOLOGY: _ **W. T. CARTER, B. S., Chie F. L. THOMAS, Ph. D., Chief; State E. H. TEMPLIN, B. S., Soi Surveyor Entomologist _ T. C. REITCH, B. S., Soil Surveyor H. J. REINHARD, B. S., Entomologist HARVEY OAKES, Soil Surveyor e B. K. FLETciiEa, M. A., Entomologist BOTANY: i W. L. OWEN, JR., M. S., Entomologist H. NEss. M. S., Chief T FRANK M. HULL, M. S., Entomologist PUBLICATIONS: i, J. C. GAINES, JR., M. S., Entomologist A. D. JAcKsoN, Chief , C. J. Tom), B. S., Entomologist SWINE HUSBANDRY: F. F. BIBBY, B. S., Entomologist _ FRED HALE, M. S., Chief S. E. McGREGOR, Ja., Acting Chief Foulbrood DAIRY HUSBANDRY: “ Inspector , Chief A. B. KENNERLY, Foulbrood Inspector POULTRY HUSBANDRY: GiLLis GRAHAM, Foulbrood Inspector R. M. SiiEnwoon, M. S., hief AGRONOMY: _ ***AGRICULTURAL ENGINEERING: E. B. REYNoLns, M. S., Chief _ _ MAIN STATlONgFARMz A. B. CONNER, M. S., Agronomist; Grain G. T. cNEss, Superintendent Sorghum Research _ _ APICULTURE, San Antonio): R. E. KARPER, B. S., Agronomist; Small Grain H. B. PARKS, . S., Chief Research v _ A. H. ALEx, B. S., Queen Breeder P. C. IVIANGELSDORF, Sc. D., Agronomist; FEED CONTROL SERVICE: in charge of Corn and Small Grain Investi- F. D. FULLER, M. S., Chief i? ations _ S. D. PEARCE, Secretary D. . KiLLouGii, M. S., Agronomist; Cotton J. H. ROGERS, Feed Inspector Breedinfi _ W. H. W000, Feed Inspector H. E. REA, ._S., Agronomist; Cotton Root Rot K. L. KIRKLAND, B. S., Feed Inspector s Investigations _ _ W. D. Noivriicurr, Jn. B. S., Feed Inspector ~ E. C. CUSHING, M. S., Assistant _in Crops SIDNEY D. REYNoLos, Jim, Feed Inspector P. R. JOHNSON, B. S., Assistant in Soils P. A. MOORE, Feed Inspector SUBSTATIONS No. I, Beeville, Bee County: No. l0, Feeding and Breeding Station, near R. A. HALL, B. S., Superintendent Colle e Station, Brazos County: R. M. HERWOOD, M. S., Animal Husband- No 2, Troup, Smith County: ‘.z>.....i|m..i.~_~_ ,...;--........az_.. 4.2“... m. Y ' - man in Charge of Farm ~ w' 3' HOTCHKISS’ Supimntendent L. J. McCALL, Farm Superintendent N“ 3- Anilcccn- Bumm‘ Cmlnt?‘ No. ll, Nacogdoches Nacogdoches County: R- H- STANSEL» M- S» S"Pc"l"tc"dc."l H. F. Moimis M. Superintendent FRANK M- HULL» M- S" Enlomolog"! "No. l2, Chillicothe, [Iardeman County: No. 4, Beaumont, Jetferson County: J. R. gumnv, B. S., Superintendent _ R. H. WYCHE, B. S., Superintendent "J. C. TEPHENS. M. A., Junior Agronomist No. 5, Temple, Bell County: _ No. 14, Sonora, Sutton-Edwards Counties: g HENRY DUNLAVY, M. S., Suliicriritendent W. H. DAnEaoN, B. S.. Superintendent B. F. DANA M. S., Plant Pat ologist E. A. TUNNICLIFF, D. V. M., M. S., H. E. REA, B._S., Agronomist; Cotton Root Rot Veterinarian _ - Investigationsi V. L. Coiw, M. S., Grazing Research Botanist i No. 6 Denton, Denton County: **0. G. BABCOCK, B. S., Collaborating “r P. DUNKLE, B. S., Superintendent Entomologist No. ‘t, SBur, Dickens County; 0. L. CARPENTER, Shepherd R. E. ICKSON‘, B. S., Superintendent No. I5, Weslaco, Hidalgo County: 5 No. 8, Lubbock, Lubbock County: W. H. FRIEND, B. S., Superintendent _ , D. L. JoNEs, Superintendent SHERMAN W. CLARK, B. S., Entomologist , FRANK GAINES, Irrigationist and Forest W. J. BAcn, M. S., Plant Pathologist Nurseryman No. l6, Iowa Park, Wichita County: No. 9, Balmorhea, Reeves County: E. J. WILSON, B. S., Superintendent _ J. J. BAYLES, B. S., Superintendent J. PAUL Lusx, S. M., Plant Pathologist Teachers in the School of Agriculture Carrying Cooperative Projects on the Station: . W. AnniANcE. M. S., Associate Professor of Horticulture . W. BILSING, Ph. D., Professor of Entomology _ . P. LEE, Ph. D., Professor of Martceting and Finance A. E., Professor of Agricultural Engineering P. Surrii, M. S.. Associate Professor of Agricultural Engineering . H. WILLIAMS, Ph. D., Professor of Animal Husbandry . K. MAcKEv, M. S., Associate Professor of Animal Husbandry . S. Mocronn, M. S., Associate Professor of Agronomy ~>wmp0 00 00 3 .w:002 00000.5 0 0.000 N 0 0 0 0.0 0.0 0.00 3 00 0 Z .m:<|0N 0000 000000000 N 050m 0.000 0 0~ 0H 0 0.0 0.0 0N0 3 00 fin I .0310 00E. 000005 0 . 0.000 0 0 0 0 3. 0.0 5 3 3 0 ~N .m0<|0N 0000 00000500 N 0800000 0.00.0 0w N0 N0 0H 00. 0.0 NA N0 N0 0N 0N .m0<|0N 0000 00000.5 0 . . . . . . .. 0 N 0 N 0N m; 0.0 0 0 0 m 33.15 00:0 000000.000 N 000030 000200 . . . . . . .. 0 0 0 N 0.0 0.0 0.5 0N 00 3 0 >370“ 0:3. 00000:. 0 0. 0. 0. R2 0.84 00mm 0002 GU02 00000.05 ©9057: QOQLOU 00.5005 0000005 0002 QUME .._0>< 4012M .0804 0003A 00.02am QUQE 000w 0000b 000005 0203K 0000005 0000 00m 0000 00m 000500 00000020000 000k. 00300010 22w 030E 000E =00 082m -0001‘ 00 00003000 00 30E 000052 QOQEDZ 003552 00385.2 0000005 00005 GQ $509k m0 .62 . 003300003 .02 000000000 0.5.5 00000000000 0.0003 w00$00o_ =0 00 00x3 00000020000 00 09$ 00a 0000000 000 0003000 0.300050 . 0 200E l0 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION on the infestations and yields is given in Table 1. Since the infesta- tions of cotton flea hoppers did not increase 0n the untreated 0r check plats t0 the extent of producing any appreciable injury, no definite data were secured on the effectiveness of the control measures applied; in other words, the differences in yields on the treated and untreated plats were not significant. The points of interest in connection with these experiments may be summarized briefly as follows: (1) When the number of flea hoppers on cotton was small the per cent of minute squares injured was not significant. (2) An increase in the number , of this insect was accompanied by a corresponding increase in the per cent of squares blasted and shed by the plants. (3) Superfine dusting sulphur proved more effective than “L” grade tobacco dust in checking infestations of the cotton flea hopper. (4) No appreciable damage re- sulted when approximately 7 per cent of the cotton plants were infested by 1 to 3 nymphs or immature insects per plant during the optimum fruiting period. The conditions which prevailed during the season of 1927 were also very unfavorable for securing definite data on the effect of any treatment applied in the experiments conducted for the purpose of determining the best time to begin dusting cotton with sulphur for the most effective control of the flea hopper. Observations in this connection were begun at all locations during May and June and continued to the middle of July. Throughout this period less than 3 per cent of the cotton plants on the treated and untreated plats in the experiment at all locations were infested with cotton flea. hoppers. The extent of injury to minute squares on the plants under observation was also very limited ranging from 0.3 per cent to 10.6 per cent and averaging approximately 5 per cent over the period of observation. These continued light infestations 0f insects were not sufficient to produce any appreciable damage to the crop and the data secured as the result of sulphur applications were not significant. Superfine sulphur, “L” grade tobacco dust, 50 :50 mixtures of sulphur- tobacco dust “L” grade, and lime-tobacco dust “L” grade were used in a series of control tests at all locations to determine the comparative effectiveness of these insecticides for controlling the cotton flea hopper. These observations extended from May 26 to August 18. As has already been pointed out above, the number of flea hoppers on the cotton during this period was too small to produce any apparent damage. These con- ditions were unfavorable for obtaining any definite information in these tests with respect to the comparative efiectiveness of the materials ap- plied for controlling the insect on cotton. Observations on the effect of “strip planting” as a measure for pro- tecting the crop from injury by the cotton flea hopper were made on a series of plats located at Taylor, Hillsboro, and McKinney, from May 28 to July 11. The number of insects during this period remained practically insignificant on the plats at all locations and no definite data were secured. INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER 11 Control Experiments at Granger, Williamson County These experiments on the control of the cotton flea hopper were located on the farm of Mr. G. C. Pope and begun on July 23, 1927. At that time there were practically no small squares on the plants; however, the lower fruiting branches were bearing bolls and large squares indicating that the cotton had not been heavily infested by the insect early in the season. The initial observations on infestation showed that approxi- mately 50 per cent of the plants on the experimental plats selected were infested by cotton flea hoppers. Superfine dusting sulphur and “L” grade tobacco dust were applied under similar conditions on separate plats to determine the effectiveness of these materials in controlling the insect. No appreciable reduction in the number of cotton flea hoppers occurred on any of the plats treated with “L” grade tobacco dust as a result of the applications made. Three plats of cotton were dusted with superfine sulphur at 5- and '7- day intervals under dry and dew conditions as indicated in Table 2. Table 2. Record of sulphur applications made on cotton at Granger Plat 1 Plat 2 Plat 3 Plat 4 July 23, 1927* July 23, 1927* July 23, 1927' 25, 1927 July 25, 1927 July 25, 1927 Date of application... . . . .. Check, no Aug. 1, 1927 July 30, 1927 Aug. 1, 1927 treatment Aug. 8, 1927 Aug. 4, 1927 Aug. 8, 1927 Interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day 5-day 7-day Dry or dew applications. . . . . . . . . . . . . . . . . . Dry_ Dry_ Dew_ applications applications applications Average rate of application ' per acre . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19.3 pounds 17.8 pounds 21 .9 pounds *Washed ofl‘ by rain, redusted July 25. Since the plants on all plats in this experiment had practically ceased growth at the time the first application of sulphur was made the reduc- tion of the infestation on the treated plats is the best index of the effectiveness of this insecticide in controlling the insect. The data se- cured in this connection are presented in Table 3. The first application of sulphur was washed off by rains within 24. hours after it had been applied and the plats were redusted on the following day. Climatic conditions during the remainder of this experiment were favorable for securing the maximum effectiveness of the insecticides applied. It will be noted that the number of insects decreased rapidly from July 23 to July 30, on all the plats dusted with sulphur. During the same period the number of insects increased approximately 20 per cent on the untreated or check plat. After the second and third applications of sulphur were made the infestation on all the treated plats was prac- tically negligible and the subsequent applications were effective in pre- N O I T A T S T N E M I R E v P X E L A R U T L U w R G A S A X E T O 8 3 O N N I T w L U B 12 dehowiaee 2E5»: :33 wuwnewaw 352a 3:0... m; o N w.o E ~ w.o o E ma, E w . . . . . . . . 1R2 AN em=w=< 0.0 w o we w E o6 w w 0.2 hm 2 . . . . . . . . 3R2 A; G325» w.o w w w.o w ~ ww .2 3 mhmm wm 3 . . . . . . . . . 1S2 N 2&5 w.N w w ow w m ow w a mam ma. flw . . . . . . . . . :5: _m ewsms< 0.: 2 E Q2 om wm Q8 NM mm wwm mm mfi . . . . . . . . . . . . BN2 .8 33.. wwm 2 w“. mam Z Nw ma». : C. ma.“ 3 N: . . . . . . . . . . . . 8N2 aw 3E. m4“? m: w: méw 2 w: mfie 2 N2 Q3 NE m: . . . . . . . . . . . . 8N2 Nu 3E. * “x535 ._. wmzmefi *Bew.afi * wmsmmi: V 3.53m we @2564 msa-EWZ 352m we $154 wAQEZ 352m we m3=w< wsa-Enz WERE we m£=w< msEEflZ .250 .6.» E60 Eel “:00 5E Ewe pom 3mm! w GE m SE w. XE . 28:8 _ 3E Eomcmpme an 82g wficoiioaxe ee eemwmwmewe~ .m view. INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER 13 venting any increase in the number of insects. In this connection it should be pointed out that natural factors also reduced the infestation on the check plat after August 3. However, the reduction in the num- ber of insects on the untreated plat occurred much more slowly than on the treated plats and did not reach the minimum which obtained on the latter. The plants on all the plats in this experiment had practically ceased fruiting at the time these observations were begun and no significant data were secured on the effect of sulphur applications in reducing the percentage of minute squares injured by the insect. On August 29, all plats averaged approximately 2.25 bolls per plant. Most of these, how- ever, had been set prior to the time when the experiment was started. The yields of seed cotton per acre secured were as follows: plat 1, (check) 480 pounds; plat 2, 522 pounds; plat 3, 522 pounds; and plat 4, 498 pounds. While all plats treated with sulphur produced a larger yield than the check or untreated plat the differences were not sufficient to be significant. The results of this control experiment may be summarized briefly as follows: (1) Sublimed velvet flowers of sulphur when applied at the rate of 20 pounds per acre is an effective insecticide for controlling an infestation of the flea hopper on cotton. (2) Applications made at 7-day intervals were practically as effective in controlling the insect as those made at 5-day intervals. (3) Applying the sulphur when the plants were wet with dew did not result in any increased effectiveness of the insecticide. Control Experiments at Hutto, Williamson County The heaviest infestation of the cotton flea hopper noted during the season of 1927 occurred on Mr. Carl Allgreen’s farm located 1 mile east of Hutto, Texas. The infestation developed late in the season in a por- tion of a large field where a poor stand of volunteer cotton had been plowed up and replanted to cotton on June 6. On August 19, when these observations were started, the older cotton in the field showed practically no injury by the flea hopper while the infestation on the young or re- planted cotton was practically complete; that is, the number of insects present was sufficient to prevent the development of any squares. There were very few bolls present at the time the first observations were made, indicating that the infestation had existed from the time that the plants were beginning to fruit. Many of the plants had made the usual tall whip-like growth as a result of the sustained cotton flea hopper infesta- tion. Another type of injury accompanied the heavy infestation of this insect. In this case the plants were deformed having a topped appear- ance with many abnormal short lateral branches bearing excessive foliage. The principal growing tips of these plants showed evidence of having been injured or destroyed some time prior to the date on which these observations were started. In this connection it should be pointed out that in 1926 a similar type of injury was noted in local cotton fields in 14 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION which there were very light infestations of the insect. However, the 1 injury was more pronounced in the heavily infested cotton at Hutto ; in f‘ fact, only a small proportion of the plants appeared normal, most of ‘; them exhibiting either one or the other types of injury described above. ‘These experiments on the control of the cotton flea hopper were begun 1 on August 19. On that date the initial observations on the extent of infestation showed that 34 to 52 per cent of the plants on the experi- mental plats selected were infested with the insect. Sublimed velvet .1 flowers of sulphur and “L” grade tobacco dust were applied at 5- and 7-day intervals under dry and dew conditions as indicated in Table 4. Table 4. Record of sulphur applications made on cotton at Hutto Plat 1 Plat 2 Plat 3 Plat 4 Aug. 26, 1927 Aug. 23, 1927 Aug. 26, 1927 Date of application... . . . .. Check, no Sept. 2, 1927 Aug. 29, 1927 Sept. 2, 1927 treatment Sept. 9, 1927 Sept. 3, 1927 Sept. 9, 1927 Sept. 8, 1927 Interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day 5-day 7-day Dew Dry or dew applications. . . . . . . . . . . . . . . . . . Dry _ . _ applications applications applications Average rate of application per acre . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.0 pounds 17. 5 pounds 17.0 pounds Climatic conditions during the extent of these experiments were favor- able for securing the maximum effect of the insecticides applied. Prac- tically no control of the cotton flea hopper was secured on any of the plats treated with “L” grade tobacco dust, although essentially the same Aug. 19, 1927 Aug. 1e, 1927 Aug. 1e, 1927 A i‘ procedure was followed in applying and noting the effect of this in- i secticide as in the experiments with sulphur described below. Effect 0f Sulphur 011 IIIfBStHtiOIII The data secured on the reduction of the number of insects as a result of dusting the plats with sulphur are presented in Table 5. From August 19 to September 5, the number of insects on all treated plats decreased steadily, while the infestation on the check increased materially during the same period. After Sep- tember 5, natural factors reduced the infestations on all plats, although the number of insects on the untreated cotton remained greater to the end of the experiment, indicating that the last applications of sulphur were producing most of the excellent control which obtained on all the treated plats on the final days of the test. _ In Figure 1 is illustrated graphically the per cent control secured on each treated plat for the duration of the test. There was no marked difference in the effectiveness of dry applications made at 5- and 7-day intervals, or dry and dew applications made at 7-day intervals. The variations in the control secured as indicated by the curves, especially after September 8, are probably due to the reductions in the infestation eflected by natural factors. 15 INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER fiopowmwcoo .235»: 5M3 c033,: 5.63 >301. 9N N w 9o w N 9N N N 9w mfi 2 . . . . . :52 .2 spnsaaw 9m N m m; w N 9N w N 9N .2 Z . . . . . 1R2 .2 HvQEQEQw 9N N; N ma. w N 9N S my NAN 2 mm . . . . .....NNNH a Hwenwfigow 9v _N w 9m NN w 93 3 NN 9Q NN S . . . . . . INNE a HBSBNQw 92 3 NN 9¢N E E, mhm 2 s“ 9% NN m: . . . . . . ...\.NN_ ._ NBEBNQm 9w». NN 3 9S NN Q. N? NN E m5 N.“ NNN . . . . . . .5: hwN a=w=< 92 S Nw 9% E 3 .93. N.» ma 92 Na wfi . . 4 . . . . . . .5: 5N ..m=w=< 9Nm S ma wen 9h. N... 9% E E 93 E N: . . . . . . . . . BN3 5N 2:34 93 N3 t. 9?. F; N2 93 c: Nw 93w N2 mm O . . . . , . . . NNZ .2 :53» Mwwmmuw.“ §3v< WNQENZ Mwwwwhmw 2.3.4 EQENZ Mmwwmwww 234 WNQENZ Mwwwmwww .532 wing/H wcvU hum wiwU 5% wEQU 5m QGQU hob 3ND w 2E m Em N Em Axufiuv _ 2E Ouuflm H6 3N3 -Q.~GQEW~QQKQ GO GOmMNQMOuGM Qfinmflh- l6 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION» Elfect of Sulphur on Squares Blasted: The reductionain the Hum of cotton fiea hoppers on all plats treated with sulphur was followed b a decrease in the number of small squares which were injured 0 blasted. The data secured in this connection are given in Table 6. August 20, more than half of the young squares on all plats were blaste as a result of the attack by this insect. The effect of the sulphur appli cations became apparent on September 5, at which time the plants on a p treated plats were beginning to retain the minute squares which were being formed rapidly. The number of squares injured by the insects on. the check or untreated plat remained high up to the final observations made on September 16, when there were approximately 50 per cent more‘ squares blasted or injured than on any of the plats dusted with sulphur.’ PERCENT 9O 80 70 60 50 40 - ----- -- v-mx ma! Armcmrors 5° s-mx max " 1_-i7-DAI DB " 20 10 AUG. 25 25 28 SRPI. 1 5 8 12 Fig. 1.——Showing the control of the flea h0pper_o_n cotton secured with sublimed flowers of sulphur at Hutto, Williamson County. Effect 0f 5111911111‘ 0H Blflflmillgi The number of blooms is an excellent index to the effectiveness of the sulphur applications which were made in this experiment to control the cotton fiea hopper. The data secured in this connection are presented in Table 7. Plats 1, 2, and 4 contained approximately the same number of plants, while the number on plat 3 was considerably larger. Calculated on the acre basis the stand of plants for each plat was as follows: plat 1, 15,532 ; plat 2, 15,233; plat 3, 16,390; and plat 4;, 15,235. It will be noted that very few blooms occurred on any of the plats from August 23 to September 9, during the time when the infestation of cotton fiea hoppers was at its maximum. About three weeks after the first application of sulphur was made, the number of blooms on all treated plats increased materially while the check plat showed no corresponding increase; in fact, the number of blooms on the check plat decreased at that time. From September 16 to October 14, the plants on all the plats dusted with sulphur were bloom- ing profusely. The check or untreated plat did not show any material lA.._.~i.._....-.¢~.— ~ ' INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER. .17 o. m5 52. 5.5 55 5.55 55 5.5m m2 . . . . . . . . . . . . . . . . . . . . . . . . . :52 52 55228522255 5.55 22 5.55 52 5.55. 22 5.255 22 . . . . . . . . . . . . . . . . . . . . . . . . . . .52 .52 55.22.252.255 5. 25 552 5.55 $2 5.55 E2 5.m5 >2 . . . . . . . . . . . . . . . . . . . . . . . . . :52 .5 55.285235 5.55 52 5.55 52 5.2 52 5.5m 5.2.2 . . . . . . . . . . . . . . . . . . . . . . . . . :52 .m 55228552255 5.55 5m2 555 552 9mm m2 5.5m 52 . . . . . . . . . . . . . . . . . . . . . . . . . . .52 .2 555852.255 5. 5 222 5.55 52 5.55 552 5.5m 552 . . . . . . . . . . . . . . . . . . . . , . . . . . . . . .552 .55 255522. 5.55 >22 o. m5 m2 5.5 522 o. 5 222 . . . . . . . . . . . ‘ . . . . . . . . . . . . . . . . .52 .5 22535 5.2% 22 5. 25 52 5.55 82 5.3 52 . . . . . . . . . . . . . . . . . . . . . . . . . .1152 .55 255522. 5. 25 mm2 5.2m 22 5.5m Q52 5.55 552 . . . . . . . . . . . _ . . . . . . . . . . . . . . . . . .52 .5 22252.4 5555 M 55.555255 55.55 5w 5.55M m 555M525 5555mm 5 555.5. 5v. 5.55225 QGQU 20mm 203G252 H220U 20mm 20.22.222.22 HGOU 20mm 208222222 220G 20mm 20322252 02mg 5 25E 5 25E 5 252.2 2525228 2 25E 25E 22525322222 .20 x5220 523 5.3553 55.25255 2252225 5a 2223 .222 2.225 55222222222 5222 222 2222 22o 2.3553 55225255 52$ 2222B 22025222250 55 525E 2.02532 22o 02225.2 o5 .3 252552222255 2522222222 5B2 22038 e23 2o 20.2.2223 22.2 92.30am d 5.352. 18 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION Table 7. The relative number of blooms on the treated and untreated plats, computed - 1 acre basis ‘Date Plat 1 Plat 2 Plat 3 Plat 4 (Check) _ August 23, 1927. . . =. . . . . . . . . , . . . . . . . . . . . . . . 103 152 152 August 26, 1927 . . . ~. . . . . . . . . . . . . . . . . . . . . . . . 413 203 109 August 30, 1927 . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 101 109 September 2, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 207 52 O September 6, 1927 . . . . . . . . . . , . . . . . . . . . . . . . 52 101 109 September 9, 1927.- . . . . . . . . . . . . . . . . . . . . . . . 258 304 164 September 12, 1927. . . . . . . . . . . . . . . . . . . . . . . . 207 354 380 ‘ September 16, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 568 1 .116 984 1 ,7 - September 19, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 155 1,012 1 .203 1,0 September 23, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 310 1 ,552 1 ,148 2, I September 26, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 861 2,888 3,499 4,6 '_ September 30, 1927 . . . . . . . . . . . . . . . . . . . . . . . . 568 2,280 2,952 3, c‘ ; October 3, 1927. . .~, . . . . . . . . . . . . . . . . . . . . . . . 1 , 188 10,236 12,460 11 ,09 October 5, 1927 . . . . . . . . . . . ... . . . . . . . . . . . . . 2,015 13,528 11,644 9,47 October 7, 1927. . .-. . . . . . . . . . . . . . . . . . . . . . .. 2,377 12,160 9,039 7,4 _ October 10, 1927 . . . . . . . . . . . . . . . . . . . . . . . . . . 4,030 17,075 19,680 16,8 _ October 14, 1927. . .; . . . . . . . . . . . . . . . . . . . . . . . 4,288 16,315 15,908 14,4 increase in thenumber of blooms until the first week in October. Un doubtedly the greater number, of blooms which occurred thereafter r the check plat was due to the fact that natural factors reduced the in festation of cotton flea hoppers after September 5 and the untreated plants also began to retain and develop squares. However, each of the treated plats had three or four times as many blooms as the check plat on the date when the final counts were made. A Effed 0f 31111111111‘ 011 F Ormsi Observations on the development of: squares, blooms, and bolls were made on all plats at weekly intervals from August 20 to October 14. The data secured are presented in Table 8. These data .further evidence the insecticidal value of sulphur in con- trolling the cotton flea hopper. At the time the first applications of sul-‘ phur were made, the plants on all plats were retaining very few squares. as a result of the injury produced by the cotton flea hopper. It will be noted that there were practically no blooms present and that the num-, ber of bolls which the plants had set was very small. This condition. remained unchanged until the first week in September when the effect. of the sulphur applications in reducing the infestation of insects became . apparent by a rapid increase in the number of squares formed on all the I cotton dusted with sulphur. The natural decrease of the number of j; insects on the check plat during September was manifested also by an 5‘ increased number of squares produced on the untreated plants. How- ever, the number and the rate of increase in the squares formed was 1 much less pronounced and occurred two or three weeks later than on the. cotton treated with sulphur. As the squares developed, the number of blooms on all the treated plats were increasing rapidly during the first two weeks in October. ‘The final observations showed that the plants j on these plats averaged more than one bloom per plant while on the ' check or untreated plat the average was slightly less than one bloom to s; every three plants. 19 INVESTIGATIONS ON CCTNTROL OF THE COTTON FLEA HOPPER wow o2 wow m5 2; $2 r5 EA m4: MZN S“ $8 . . . . . . . . . .52 .3 5.330 N? m. 82 8m 1.. NHL :3 Nm~ mwmfl m: 5 5w . . . . . . . . . BN2 R x5800 m: hm mmfi w»: 2 $2 N: fiN m9: mw w .5“. . . . . . . . BN2 d» Laifizaww m2 wN $3 ow m $2 ow oN “$2 E. w mmN . . . . . . . BN2 .wN Qvnfiuwavw S. 2 wwn E 2 8w 3 2 wwo mm w w: . . . . . . . 6N2 A: 58530.” 3 fi 3w om w fim w». m 3w mm N 5 . . . . . . ihNi d hwfifivfiww Nv fi wN 5 o mm $ ~ Nw mm w flw . . . . . . . 8N2 .N “Qaafigow Q. m “a hv N 2w fi m 5 m5 w Nw . . . . . . . . . . 8N2 6N fi=m=< Q N Nm 3 w i. Nm. m 3 mm w oNfi . . . . . . . . . . 6N2 .oN @2654 mmnmzom miofim mobwnvm 23m mEOO-m wohwsvm 23m miofim wuuwsdm mzom mfioflm wobwdvw 8RD v Em m Em N GE Axnsauv fl RE $33 wvaauhwc: v.3 wowmob no 23m 1am $82.3 @932.» mo QQQEQO~Q>QU ucw doflonwoum .w o-nwnr 20 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION Elfect of Sulphur 0n Fruiting= In Table 8 it will be noted that mo of the bolls were set after October 1. Obviously this was too late in the season for these bolls t0 mature and hence yields are not available to de- termine the effectiveness of sulphur in controlling the insect. In th connection, however, the number of bolls set by the plants on all the plats is a good basis for comparison. An increase in the number, of, bolls set by the plants on all treated plats became apparent on September 23, approximately four weeks after the first application of sulphur was made. Each record made thereafter showed a heavy increase in the number of bolls on the treated cotton. On October 14, when the last, observations were made, the untreated cotton averaged 1.4 bolls per= plant while the cotton dusted with sulphur averaged 4.2 bolls per plant.“- In other words, there were three times as many bolls set on the cotton which was dusted with sulphur to control the cotton flea hopper. a The significance of these results may be emphasized by pointing out‘ that the experiment was conducted late in the growing season when the Q: boll weevil destroyed many forms on the plants under observation, and that a practically complete infestation of cotton flea hoppers had pre- _ vailed and destroyed a large proportion of the squares formed on the » cotton for some time prior to August 19, when the first applications of l, sulphur were made. f The results secured in this control experiment on the cotton flea ‘ hopper may be summarized briefly as follows: (1) A complete in- i, festation of this insect on cotton was efiectively controlled by applying VT sulphur dust at the rate of 1'7 pounds per acre. (2) Applications of f this insecticide made at 5- and 7-day intervals during the daytime and it at 7-day intervals early in the morning when the plants were wet with ‘ dew, all proved efficient in reducing the number of insects. (3) The 1i reduction in the number of insects on all treated plats was followed by a ' decrease in the per cent of minute squares injured or blasted. (4) ; The increased number of small squares retained by the sulphured plants . became apparent about three weeks after the first application was made. _ (5) Four or five applications of sulphur materially increased the num- » her of blooms on all treated plats. (6) q The number of bolls set on the cotton dusted with sulphur was approximately three times greater than the number set by the plants which were not treated. . sum,‘ EXPERIMENTS ON THE CONTROL OF THE COTTON FLEA HOPPER ON GOATWEEDS To further note the insecticidal value of the various materials used in controlling the cotton flea hopper and to secure further information regarding the most effective time and frequency of application of in- secticides, fifteen triplicate control tests on this insect were conducted on Uroton, or goatweed, during August and September, 1927. Each series of these control tests extended over a period of two weeks and was conducted on four one-eighth-acre plats. One plat of goatweeds INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER 21 in each series was left untreated to serve as a check on the effectiveness . of the insecticides applied on the other three plats. Daytime or dry applications of insecticides were made at 5- and 7-day intervals, and early morning 0r dew applications at 7-day intervals. All of the in- secticides were applied uniformly over the area of each plat at the rate of 15 to 20 pounds per acre with hand-operated Niagra dusting machines. Prior to the first application and each day thereafter during the extent of the tests fifty terminal bud-clusters were removed from plants situ- ated on all portions of both treated and untreated plats. These samples were placed in tight containers and taken to the laboratory where the insects present on the bud-clusters were counted carefully to determine» the extent of infestation. The number of insects present on the bud- clusters taken from the untreated plats was considered to be a 100 per cent infestation and the daily control secured on all plats was computed on that basis. Climatic conditions during these control tests were favorable for main- taining the maximum effect of all insecticides applied. The tempera- tures were high throughout August, 1927, and the rainfall for the month totaled 0.01 inch. These conditions were unfavorable for plant growth and the weeds on some plats deteriorated before the tests were com- pleted. In effect, this naturally reduced the number of cotton flea hoppers on the plats affected most by the drought, and the calculations on the per cent of daily control obtained on these plats were based upon comparatively small numbers of insects, which in some instances averaged.’ less than one insect per plant. Undoubtedly the unusually high control of insects secured on these plats is attributable in part to the reduction in infestation produced by natural factors. Tests on Time and Frequency of Application Nine triplicate control tests on the cotton flea hopper were conducted. to determine any difference in efiectiveness of dry and dew applications of insecticides and also to secure data on the comparative effectiveness of applications made at 5- and 7-day intervals. During these control tests dews were very light and often hardly noticeable. However, conditions for applying the insecticides in the» early morning were more favorable since there was practically no» aiir; movement at that time. Strong winds often interfered with securing: uniform applications during the mid-day or afternoon. A summary of]. the data secured in these tests is presented in Table 9. These data indi- cate that applications of insecticides made early in the morning at 7-day intervals are more effective in reducing the infestation of insects than the applications made later in the day at the same intervals when winds. _ interfered. As has already been pointed out, the dews were very light: or practically insignificant at the time these tests were conducted and‘. the increased effectiveness of the early morning applications apparently- was due to the more favorable conditions for applying the dust. These- _mixtures than on those treated with the straight sulphurs. ‘Z2 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION data also indicate that applications of insecticides made at 5-day inter- , vals when the plants are dry produce a higher control of the cotton flea; hopper than those made at weekly intervals. The increase in per cent . of control was more apparent on the plats dusted with sulphur-tobacco However, a this was due, in part at least, to the more rapid deterioration of the 1 plants on all plats dusted with the sulphurs, which effected a natural l reduction of the number of insects present. i : The effect of time and frequency of application of insecticides on goatweed for the l Table 9. control of the cotton flea hopper _ _ Per Cent Insecticide Schedule of Average Application Daily Control Sulphur-Tobacco Dust “L” grade, 60:40 mixture . . . . . . . . . . . . . 7-day, dry 46.9 Sulphur-Tobacco Dust,“L” grade, 70:30 mixture . . . . . . . . . . . . . 7-day, dry 74.7 Sulphur-Tobacco Dust,.“L” grade, 80 :20 mixture . . . . . . . . . . . . . 7-day, dry 73.3 Sulphur-Tobacco Dust_ “L” grade, 60:40 mixture . . . . . . . . . . . . . 5-day, dry 72.4 Sulphur-Tobacco Dust,“L” grade, 70:30 mixture . . . . . . . . . . . . . 5-day, dry 71.9 _5iilphur-Tobacco Dust,“L” grade, 80:20 mixture. . .. . . . . . . . . . 5-day, dry 84.4 Sulphur-Tobacco Dust;.“L” grade, 60:40 mixture . . . . . . . . . . . . . 7-day, dew 72.0 Sulphur-Tobacco Dust"‘L” grade, 70:30 mixture . . . . . . . . . . . . . 7-day, dew 87.6 Sulphur-Tobacco Dust “L” grade, 80:20 mixture . . . . . . . . . . . . . 7-day, dew 77.2 Sulphur-Tobacco Dust 3% Nicotine, 60:40 mixture . . . . . . . . . . . . . 7-day, dry » 58.3 ulphur-Tobacco Dust 3% Nicotine, 70:30 mixture . . . . . . . . . . . . . 7-day, dry 60.0 Sulphur-Tobacco Dust 3% Nicotine, 80:20 mixture . . . . . . . . . . . . . 7-day, dry 56.2 Sulphur-Tobacco Dust 3% Nicotine, 60:40 mixture. . . . . . . . . . . . . 5-day, dry 70.2 Sulphur-Tobacco Dust 3% Nicotine, 70:30 mixture . . . . . . . . . . . . . 5-day, dry 77.0 Sulphur-Tobacco Dust 3% Nicotine, 80:20 mixture . . . . . . . . . . . . . 5-day, dry 72.2 Sulphur-Tobacco Dust 3% Nicotine, 60:40 mixture. . . . . . . . . . . 7-day, dew 64.1 Sulphur-Tobacco Dust 3% Nicotine, 70:30 mixture . . . . . . . . . . . . . 7-day, dew 73.6 Sulphur-Tobacco Dust 3% Nicotine, 80:20 mixture . . . . . . . . . . . . . 7-day, dew 79.2 ulphur, Superfine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 7-day, dry 81.9 Sulphur, Swan No. 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day, dry 91.4 Sulphur, Electric . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day, dry 88.8 Sulphur, Su erfine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-day, dry 96.0 Sulphur, Ve vet flowers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-day, dry 97.4 Sulphur, Electric . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-day, dry 97.3 Sulphur, Su erfine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day, dew 94.5 Sulphur, Ve vet flowers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day, dew 90.0 Sulphur, Electric . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-day, dew 88.7 Tests on Comparative Elfectiveness of Insecticides ' From August 20 to September 3, six series of triplicate tests were conducted for the purpose of comparing the effectiveness of several in- secticides in controlling the cotton flea hopper. Each series of these ‘tests extended over a period of fourteen days and included two dry or daytime applications of insecticides at 7-day intervals. A summary of the data on the per cent of daily control secured in these tests is presented in Table 10. It will be noted that the effectiveness of these insecticides varied greatly in controlling infestations of the cotton flea hopper. Seven out of the nineteen difierent materials used resulted in an excellent control of the insect. These include the four brands of sulphur which were used; two sulphur-tobacco dust “L” grade mixtures; and the mixture of 23 INVESTIGATHTNS ON’ CfiNTROL OF THE COTTON FLEA HOPPER 3 3 m.mm c.c» c.m» 3.c» ».3.m ».mm 93m 32cm ».cm 9mm m.cc 93.. cmc 93c 9c3 . . . . . . . . . .........2§mB 35m 05.3022 0cm 25G ovocnoha3sca3sm 3 c3 9cm c.mm m. c» 9c» m.m» c.»m c. 3m 9cm 9»3. 9 3m 3mm 93m 9mm 3.3m 9cc . .. ......5..EE ems... ...m.._=w-....§s.=< EBBQO 3 m3 13 9m3. 933. 9c3. 9cm 9»3. ».c3. mmm 9mm ».33. 93. 9mm c.»3. m.mm 9mm ................2.€2z§ 335 0.3.63. 3 m3 c.»m 93m 93. 93“ 3mm 93m 9cm 9mm 9mm #2.. ».»3. 93“ 9cm 9cm mcc . . . . . . . .........:%§..31.533 Q2303. 3 » 9c» ».3m 9mm 3.3m c.»m 9»m c.mm m.»» 9cm 9mm 9cm 9»» ».c» 9cm 93. . . . . . ....2=ama Qwomé vfifibmcmm :3: ¢Q¢§3o,3..._=mc3cm 3 c3 32cm 3.. mm 9mm 9mm 32cm 93m c. mm 9mm 9mm c. mm 9m» 9mm m. 3m 9mm ». mm . . . . 5255.8 23m oafiafic. =3: oQQBoE 3 c3 93 9c m3. ».m3 ».»c 9m3 9c3 933 3.c 93 9m3 933 9cm Ia 93. ...Q._E.m3E 3.2a oasozz Qcm 333G ovvcnob-bzcxsm 3 »3 Qfi. 9cm 3.m3. 9m3. 32cm c.3m ».mm 9mm 9c3 m..m3 mmc 9mm 93m 93. 93m ...................:B.ma 3...... @5332 emvm c253 oo¢§3¢HL=ma3=w c3. c 9.3 9mm 3.3m 3m» 9m» m.»» 93.» ».»m 9mm 93m c.3m ».mm 9mm 93m ».mm ..................@.:s.x3E 23¢ . wEmoBZ mcm 32D oc$no,3...==3n3=w c3 c m.c» 9cm 9mm 9m» m.3m 9cm mmc 93c ».mm 9mm 9mc 9cm is 9cm c.3» . mssom $22, .525 mm 3 ».mm 9mm mcc 9mm 3.cc cmc cmc mcc ».c» 9m» 9mm m.cc 9cm 9cm m.m» ..............asa._m 53am 3 c 93m ».3.m mmm 3mm mcc Q; 93c 93c 3.»» 9mm ».mm 3mm m.cc c.»» m.mm dZ 5am émnzw 3m m 9cm m.»» 3mm» 9cm 9»m ».3c 93c 93m ».mm 3......» m.»» 3.3m ».3.m c.m» 32cm ..2..E&.m 5225 c3 m3 9cm 3m» 3.3m 93m ».»» m.mm m.3.m 93m 9c =3 9mm 93» 9cm 9»m 93m , . . . . . . . . . . .......@.:E:.E .63 $235032 Qcm i=5 oeocnokénnnxsw c3 33 c.3m m.cm ».mm 9mm ».m» 93c 9cm 9c» 9m 93m 3.»m mc» 9cm is 9cm , . . , . . ;..........2a..ma $3» Eméz c” :5 sgoaazmczw 2 m Ts <3 Ia fa o3 I.» 2w i» Q2 i: ca. vs Z... i» m? . . . . . . . ..............3.ma 2% vammoumz $3.“ c263 OooanbPLflAQ-iw c3 m 93.» 93m 9c» 9mm 9mm 9mm 9cm 9mm c.»» 93.» m.»» 9cm 3.3.» m.»» 9»3. . . . . . . . . ..........~.:<.E3E =2; , 2.2m :3: c253 ooucnckhsmn3aw 2 m w: 93» ._...$ f: c»... ca i» f; Q2 i: I: m2 i: we 3m .......... .......2s.me =2: umfim =3: i=9 eognokLsmmiw c3 m3 m.cm 3.»c mmc 9mm 93. 9cm 3.3m 9c» m.mm 93.3. 9mm 9mm c.m» 9mm 9mm . . . . . . . _..........33na 2.5m 03.2w ...3_. i=0 8$m¢31=ms=w Q59? 3055.0 x630 3:33 cmwfl QQ .3333 .933 E5 5R3 QQ TO .30 .95 .333 5.53 .533 >33 .333 8m§muvwn3 can 5o 53 £3 .333 fic3 £33 .383 m; 5m E» 5m 5m £3. 3cm mac i3 . mo .OZ 3~._...>< uoQE-ucm no .6350: cam nommou v.3... mnfiobnou 6.3 3m»: E cow: 83.329633 233 mo M352 3:8 mmwmksuuomw mo wmcmaouba mackomm smcfl .c3 @393. 24 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION sulphur-tobacco dust-lime. The performance of the sulphurs in reduc- ing the number of insects is especially noteworthy. Each brand of sulphur used resulted in a very rapid reduction in the infestation and ” remained effective in checking the multiplication of the insects through- p’; out the duration of the tests. On the basis of the average per cent of I daily control produced the sulphurs rank closely together ranging from 79.8 to 86.7 per cent. These differences are not considered significant F and the cost of the material is the determining factor regarding the kind 1 or grade of sulphur to use in combating outbreaks of the cotton flea ; hopper. Many requests have been received for information regarding the pos- sibility of controlling the cotton flea hopper and the boll weevil simul- 5 taneously with a mixture of sulphur and calcium arsenate. To deter- mine the effectiveness of a combination insecticide for controlling the cotton flea hopper a mixture containing 5O parts sulphur and 50 parts 5 calcium arsenate was applied on a plat of goatweeds infested with the insect. The results secured are presented in Table 10, and indicate that this combination insecticide is decidedly less effective than un- diluted sulphur in controlling infestations of the cotton flea hopper. An f average daily control of 59.3 per cent was secured by the use of this 3 mixture while the undiluted sulphurs applied under comparable condi- j tions produced an average daily control ranging from 79.8 to 86.7 per cent. In the light of these results and the control secured in experi- ments on the boll weevil it is not considered that applications of this combination insecticide would be warranted even though both insects may be present. The results of several preliminary tests reported in Texas Station Bulletin 356 indicate that mixtures of sulphur and tobacco dust were slightly more effective than straight sulphur in controlling the cotton flea hopper. To secure additional information on this point seven differ- ent mixtures of sulphur-tobacco dust and two grades of undiluted to- bacco dust were applied on twelve separate plats of infested goatweeds. The results secured in these tests are given in Table 10. Under the conditions of these tests none of the materials produced the high con- trol of the insect that was secured on the plats dusted with the sulphurs. In fact, only the 70 :30 and 80 :20 mixtures of the sulphur-tobacco dust “L” grade resulted in a uniform reduction of the number of insects present on the treated plats throughout the duration of the tests. The results of these tests on the control of cotton flea hopper may be summarized briefly as follows: (1) Early morning or dew applications of insecticides are slightly more effective in controlling the cotton flea hopper than daytime or dry applications. (2) Insecticides applied at 5-day intervals were most efiective in reducing the number of insects present, but a satisfactory control was also secured by applications made at 7-day intervals. (3) Electric sulphur, Superfine ventilated sulphur or Swan brand No. 1, Superfine dusting sulphur, and Sublimed velvet INVESTIGATIONS ON CONTROL OF THE COTTON FLEA HOPPER 25 flowers of sulphur proved to be the most effective of all the insecticides used in the control tests on the cotton flea hopper. ACTION OF SULPHUR ON THE COTTON FLEA HOPPER Several tests were conducted to determine the action of sulphur on the cotton flea hopper. Individual goatweed plants were selected in the field and dusted liberally with sulphur while exposed to the direct sun- light during the hottest portion of the day. Superfine sulphur was applied in the first test at 2 :05 o’clock p. m. on August 25, 1927. The day was hot and sultry, the sky partly cloudy, and the sun frequently obscured for brief periods. There was practically no air movement during the time the observations were made. The temperatures taken 20 inches above the surface about on a level with the plant ranged from 98 to 104 degrees F.; averaging 101.8 degrees F. during a 2-hour period, from readings taken at 10-minute intervals. The effect of the sulphur on the insects first became apparent 42 minutes after it was applied, at which time the youngest nymphs began dropping to the ground and died shortly thereafter. The older nymphs were also beginning to exhibit discomfort at this time and moved about as if seeking relief. The first fatality among the insects which were one-half or more developed was noted at 2:57 o’clock or 52 minutes after the sulphur was applied. Thereafter most of the youngest nymphs on the plant died rapidly, although there were several fifth instar individuals alive on the plant two hours after it had been dusted with sulphur. Twenty-four hours later the plant was again carefully examined and only one live fourth instar nymph was found. These observations indicate that sulphur applied under favorable conditions becomes effective quickly and is a very eflicient insecticide for killing the immature stages of the cotton flea hopper. Throughout the duration of these tests the adult cotton flea hoppers on the plant were not noticeably affected by the application of sulphur. Many were observed to fly away as the insecticide was being blown over the plant with a hand-operated dusting machine. However, a number of the adult insects remained on the sulphured plant for at least two hours without showing any indication of being affected unfavorably either by coming in contact with or by the fumes given off by the sulphur. Sublimed velvet flowers of sulphur was used in a test similar to that described above. The results secured were essentially the same; that is, in about one hour after the sulphur was applied to the plant a large proportion of the young cotton flea hoppers were killed by the effects of the insecticide. Twenty-four hours after the application of sulphur had been made, an examination of the plant disclosed no live nymphs although adult cotton flea hoppers were hardly less abundant than on the adjacent untreated plants. 26 BULLETIN NO. 380, TEXAS AGRICULTURAL EXPERIMENT STATION OBSERVATIONS ON FOOD PLANTS During the course of these investigations several food plants of the; cotton flea hopper, not heretofore recorded, were encountered in the field during May, 1927. These include the following five species of weeds collected at Robstown, Wharton, Taylor, and College Station: 3 Scientific Name Common Name Abutvllon myalacwn . . . . . . . . . . . . . . . . . . . . .Indian Mallow pGaillamdia pulchella . . . . . . . . . . . . . . . . . . . .-—-—i—- Gaum brachycarpa, . . . . . . . . . . . . . . . . . . . . _Primrose Gaura, Pwltcheri . . . . . . . . . . . . . a . . . . . . . . . . .Primrose Marrubiuvn vulgare . . . . . . . . . . . . . . . . . .. Hoarhound Additional food plants of the cotton flea hopper are reported in Texas Station Bulletins 339 and 356. Among the food plants listed above, Gaura brachycairpa is also known to be an important factor in the hiber- a ‘ nation of the insect (Texas Station Bulletin 377) in the vicinity of '11" Wharton, Texas. Although the present known food plants of this insect 1 include 59 different species, principally common weeds, there are un- f doubtedly many other plants, not yet discovered, which are also im- i portant in this connection. Since the insect feeds upon a large variety of early spring weeds com- f monly found in cotton fields, the complete destruction of all weed growth several days prior to planting cotton is a desirable procedure in com- . bating early infestations of the insect. SUMMARY During the early part of the season in 1927 infestations of the cotton flea hopper were generally light on cotton and very little injury by this insect was noted. During July and August rather severe infestations of the flea hopper were found in cotton fields located near Granger and Hutto, Texas. ~ Control experiments were conducted on half-acre plats in these in- fested fields using “L” grade tobacco dust and sublimed velvet flowers of sulphur. The applications were made at 5- and 7-day intervals un- 1' der dew and dry conditions at the rate of 15 to 20 pounds per acre. No satisfactory control of the cotton flea hopper was secured by the use of “L” grade tobacco dust. Sublimed flowers of sulphur applied either in the early morning or during the daytime proved very effective in reducing infestations of the insect. The reduction in the number of insects on all treated plats was followed by a material decrease in the number of minute squares in- jured or blasted. The increased number of small squares retained by the sulphured plants became apparent about three weeks after the first application was made. Four or five applications of sulphur greatly increased the number of blooms on all treated plats. On the final date INVESTIGATIONS ON CONTROL OF'THE COTTON FLEA HOPPER 27 of observation the untreated cotton averaged 1.4 bolls per plant; the sulphured cotton 4.2 bolls per plant. The bolls were set too late in the season to mature and the comparison of yields of the treated and check plats could not be secured. Nineteen different insecticides were used in a series of tests to deter- mine the effectiveness of these materials for controlling infestations of the cotton flea hopper on goatweed. All insecticides were applied at 7- day intervals during the daytime at the rate of 15 to 20 pounds per acre and each test extended over a period of 14 days. Climatic conditions were favorable for securing the maximum effect of the insecticides used in all tests. ~ Electric sulphur, Superfine ventilated sulphur, Superfine dusting sulphur, and Sublimed flowers of sulphur proved the most effective in- secticides among the materials used for controlling the cotton flea hop- per. The average daily control secured with these grades of sulphur in all tests over 14-day periods ranged from 79.6 to 86.7 per cent. Two mixtures of sulphur-tobacco dust “L” grade, viz., 70:30 and 80:20, re- sulted in an average daily control of 71.1 and 74.2 per cent, respectively. In nine series of tests insecticides applied at 7-day intervals early in the morning under conditions of very light dews produced a slightly higher control of the insect than daytime applications of the same ma- terials made at 7-day intervals. Applications of insecticides made at 5- day intervals were most effective although an average daily control of 8O per cent was secured with materials applied at 7-day intervals under favorable climatic conditions. Sulphur applied under conditions of little or no air movement during hot clear days affects the young cotton flea hoppers very quickly. Ob- servations in this connection show that many nymphs are killed within an hour from the time of applying the insecticide. The adult or matured insect apparently is not aifected unfavorably by the insecticide applied under field conditions. Five new food plants of the cotton flea hopper are listed. These comprise common species of spring weeds collected at Robstown, Whar- ton, Taylor, and College Station, Texas.