LIBRARY, A 8n M COLLEGE» CAMPUS. A98-1030-10M-L180 TEXAS AGRICULTURAL EXPERIMENT STATION A. B. CONNER, DIRECTOR COLLEGE STATION, BRAZOS COUNTY, TEXAS BULLETIN NO. 419 , l ’ DECEMBER, 1930 DIVISION OF HORTICULTITRTEV <3 v '7’ ,1» . Citrus Production in the Lower Rio Grande Valley of Texas AGRICULTURAL AND MECHANICAL COLLEGE OF TEXAS T. O. WALTON, President STATION STAFFT ADMINISTRATION: A. B. CONNER, M. S., Director R. E. KARPER, M. S., Vice-Director CLARIcE MIxsON, B. A., Secretary . P. HOLLEMAN, JR., Chief Clerk J. K. FRANOKLOW, Assistant Chief Clerk CHEsTER HIGGS, Executive Assistant C. B. NEBLETTE, Technical Assistant CHEMISTRY: G. S. FRAPs, Ph. D., Chief; State Chemist S. E. AsRURY, M. S.. Chemist J. F. FUDGE, Ph. D., Chemist E. C. CARLYLE, B. S., Assistant Chemist WALDO H. WALKER, Assistant Chemist VELMA GRAHAM, Assistant Chemist T. L. OGIER, B. S., Assistant Chemist ATHAN J. STERGES, B. S., Assistant Chemist JEANNE M. FUEOAs, Assistant Chemist RAY TREIcHLER, M. S., Assistant Chemist RALPH L. ScHwARTz, B. S., Assistant Chemist C. M. POUNDERs, B. S., Assistant Chemist HORTICULTURE: S. H. YARNELL, Sc. D., Chief L. R. HAWTHORN, M. S., Horticulturist RANGE ANIMAL HUSBANDRY: J. M. JoNEs, A. M., Chief B. L. WARwIcK, Ph. D., Breeding Investigations STANLEY P. DAvIs, Wool Grader ENTOMOLOGY: F. L. THOMAs, Ph. D., Chief; State Entomologist H. J. REINHARD, B. S., Entomologist R. K. FLETcHER, Ph. D., Entomologist W. L. OWEN, JR., M. S.. Entomologist J. N. RONEY, M. S., Entomologist J. C. GAINEs, JR., M. S., Entomologist S. E. JoNEs, M. S., Entomologist F. F. BIBBY, B. S.. Entomologist CECIL E. HEARD, B. S., Chief Inspector OTTO MAcKENsEN, B. S. Foulbrood Inspector é . B. WHITNEY, Foulbrood Inspector AGRONOMY: E. B. REYNOLDS, Ph. D., Chief R. E. KARPER, M. S., Agronomist P. C. MANGELsDORE, Sc. D., Agronomist D. T. KILLOUOH, M. S., Agronomist . E. REA, B. S., Agronomist —>——————~—-—, Agronomist _ . C. LANGLEY, B. S., Assistant in Soils PUBLICATIONS: A. D. JAcKsON, Chief VETERINARY SCIENCE: *M. FRANcIs, D. V. M., Chief H. ScHMIDT, D. V. M., Veterinarian F. P. MATHEws, D. V. M., M. S., Veterinaria. W. T. HARDY, D. V. M., Veterinarian F. E. CARROLL. D. V. M., Veterinarian PLANT PATHOLOGY AND PHYSIOLOGY: J. J. TAUBENHAUS, Ph. D., Chief W. N. EzEKIEL, Ph. D., Plant Pathologist W. J. BAcH, M. S., Plant Pathologist B. F. DANA. M. S., Plant Pathologist FARM AND RANCH ECONOMICS: L. P. GABBARD, M. S., Chief W. E. PAULsON, Ph. D., Marketing C. A BONNEN, M. S., Farm Management W. R. NISBET, Ranch Management Specialia i———————, Assistant RURAL HOME RESEARCH: JEssIE WHITACRET, Ph. D., Chief MARY ANNA GRIMEs, M. S., Textiles ELIZABETH D. TERRILL, M. A., Nutrition SOIL SURVEY: **W. T. CARTER, B. S., Chief E. H. TEMPLIN, B. S., Soil Surveyor A. H. BEAN, B. S., Soil Surveyor R. M. MARsHALL, B. S., Soil Surveyor BOTANY: V. L. CORY, M. S., Act. Chief SIMON E. WOLFF, M. S., Botanist SWINE HUSBANDRY: FRED HALE, M. S., Chief DAIRY HUSBANDRY: O. C. COPELAND, M. S., Dairy Husbandman POULTRY HUSBANDRY: . M. SHERWOOD, M. S., Chief AGRICULTURAL ENGINEERING: H. P. SMITH, M. S., Chief MAIN STATION FARM: G. T. McNEss, Superintendent APICULTURE (San Antonio): H. B. PARKS, B. S., Chief A. H. ALEX, B. S., Queen Breeder FEED CONTROL SERVICE: F. D. FULLER. M. S., Chief . D. PEARcE, Secretary . H. ROGERS. Feed Inspector . L. KIRKLAND, B. S., Feed Inspector . D. NORTHcUTT, JR., B. S., Feed Inspectoi DNEY D. REYNOLDS, JR., Feed Inspector . A. MOORE, Feed Inspector E. J. WILsoN, B. S., Feed Inspector w@zx~w SUBSTATIONS N 1, Beeville, Bee County: o. R. A. HALL, B. S., Superintendent No. 2, Troup, Smith County: P. R. JOHNSON, M. S., Superintendent No. 3, Angleton, Brazoria County: R. H. STANsEL, M. S., Superintendent No. 4, Beaumont, Jefferson County: R. H. WYcHE, B. S., Superintendent No. 5, Temple, Bell County: _ HENRY DUNLAVY, M. S., Superintendent B. F. DANA, M. S., Plant Pathologist H. E. REA, B. S., Agronomist; Cotton Root Rot Investigations _ _ SIMON E. WOLFF, M. S., Botanist;_Cotton Root Rot Investigations No. 6, Denton, Denton County: P. B. DUNKLE, B. S., Superintendent No. 7, Spur, Dickens County: _ R. E. DICKSON, B. S., Superintendent -————————i, Agronomist No. 8, Lubbock, Lubbock County: D. L. JoNEs, Superintendent FRANK GAINEs, Irrigationist and_Forest Nurseryman _ No. 9, Balmorhea, Reeves County: J. J. BAYLEs, B. S., Superintendent No. 10, College Station, Brazos County: R. M. SHERWOOD, S., In charge L. J. McCALL, Farm Superintendent No. 11, Nacogdoches, Nacogdoches County: H. F. MORRIs, M. S., Superintendent **No. 12, Chillicothe, Hardeman County: J. R. QUINRY, B. S., Superintendent **J. C. STEPHENS, M. A., Assistant Agronomist N0. 14, Sonora, Sutton-Edwards Counties: W. H. DAMERON, B. S., Superintendent ——-——————, Veterinarian W. T. HARDY, D. V. M., Veterinarian **O. G. BABCOCK, B. S., Entomologist O. L. CARPENTER, Shepherd No. 15, Weslaco, Hidalgo County W. H. FRIEND, B. S., Superintendent _ SHERMAN W. CLARK, B. S., Entomologist \V. J. BAcH, M. S., Plant Pathologist No. 16, Iowa Park, Wichita County: N C. H. McDOwELL, B. S., Superintendent o. 17, ———————--—-- No. 18, ———-———, Superintendent No. 19, Winterhaven, Dimmit County: E. MORTENsEN, B. S., Superintendent N L. R. HAWTHORN, M. S.,‘Horticulturist 0. 20, ——-—-—————— , Superintendent —, ‘Superintendent Teachers in the School of Agriculture Carrying Cooperative Projects on the Station: W. ADRIANcE, Ph. D., Horticulture W. BILSING, Ph. D., Entomology _ P. LEE, Ph. D., Marketing and Finance ScOATEs, A. E., Agricultural Engineering . K. MAcKEY, M. S., Animal Husbandry *Dean School of Veterinary Medicine. **In cooperatlon wIt J. S. MOGFORD, M. S., Agronomy F. R. BRIsON, B. S., Horticulture TIN. R. HORLAcHER, Ph.-D., Genetics H. KNOX, M. S., Animal Husbandry TAs of December 15, 1930. h U. S. Department of Agriculture. Citrus fruit production in the Lower Rio Grande Valley, ‘especially grapefruit, has increased at a rather rapid rate dur- ing the past few years. More than 5,000,000 citrus trees were set in orchard form in the Lower Rio Grande Valley up to July, 1929. The proportion of the acreage which is being set to grapefruit indicates that growers and shippers have found the grapefruit to be the most profitable type of citrus fruit for this region. Of the grapefruit varieties now available, Marsh and Thompson are obviously the most desirable types for com- mercial planting. Sweet oranges are apparently not as well adapted to local conditions as are grapefruit but are being grown to a limited extent. Until a variety of sweet orange is developed or introduced which will combine early maturity and good “keeping quality” with excellence of flavor and prolific bearing capacity, this industry will not keep pace with grapefruit production. The problem of root stocks for citrus in the Lower Rio Grande Valley is not a problem of major importance at the present time. The commonly used sour-orange stock appears to be a very desirable type for use in propagating most of the commercial forms. The soils on which citrus are usually grown in the Lower Rio Grande Valley are very fertile, and experiments with fer- tilizers on the Victoria fine sandy loam soil, up to the present time, indicate that soil fertility has not become a limiting factor in grapefruit production, under the conditions of these experiments. However, these results should not be interpreted as meaning that fertilizer should be withheld after the trees reach bearing age. Moderate applications of fertilizer along with other beneficial orchard practices, throughout the develop- ment and maintenance of the orchard, will tend to keep the original fertility of the soil unimpaired. As citrus fruits are grown under irrigation in this region, the physical nature of the soil must be given due consideration. Leguminous cover cropping and mulching has been found to exert a beneficial effect on the soil as shown by the increased production from plats where these practices were followed. It seems desirable to recommend a system of seasonal, legu- minous cover cropping, rather than the usual method of clean cultivation with only sporadic natural cover crops. Plowing the soil to a depth of six inches once each season was not found to be of practical value. " . The size attained by Valley grapefruit trees makes the close spacing practiced in some of the older citrus-producing areas impracticable in this region. Apparently a spacing distance of 25x25 feet is desirable with grapefruit trees in the Lower Rio Grande Valley. CONTENTS PA i‘ Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . l Soils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 . . . . . Climate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Scope of Publication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. Citrus Variety Standardization . . . . . . . . . . . . . . . . . . . . . . . . . . Plan of Standardization Experiment . . . . . . . . . . . . . . . .. y’ Explanation of Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 9 Interpretation of Results . . . . . . . . . . . . . . . . . . . . . . . . . .. 181 Grapefruit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 18? Grapefruit Quality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 19< Standards for Maturity . . . . . . . . . . . . . . . . . . . . . . . . . . .. 20f Description of Grapefruit Varieties . . . . . . . . . . . . . . . . . . 20 Sweet Orange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 22 Sweet Orange Quality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Description of Sweet Orange Varieties . . . . . . . . . . . . . .. 24 Mandarin and Tangerine Orange . . . . . . . . . . . . . . . . . . . . . . .. 26 King and Satsuma Orange . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 26 Lemon and Lime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 26 Kumquats, Hybrids, and Miscellaneous Forms . . . . . . . . . . . . . 28 Citrus Root-Stocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Plan of Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Interpretation of Results. . ~. . . . . . . . . . . . . . . . . . . . . . . . . 30 Grapefruit Orchard Management . . . . . . . . . . . . . . . . . . . . . . .. 34 Orchard Plat Technic . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 35 Cultivation, Cover Crops, and Mulches . . . . . . . . . . . . . . . . 44 Plan of Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . .. 44 Interpretation of Results . . . . . . . . . . . . . . . . . . . . . .. 45 Fertilizer Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 48 Plan of Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . .. 48 Interpretation of Results . . . . . . . . . . . . . . . . . . . . . .. 52 Spacing of Grapefruit Trees . . . . . . . . . . . . . . . . . . . . . . . . . . .. 55 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 55 Literature Cited . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .V . . . . . . . 58 BULLETIN‘ NO. 419 DECEMBER, 1930 CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS HAMILTON P. TRAUB* AND W. H. FRIEND The preliminary experiments concerning citrus fruit produc- tion at Substation N0. 15, Weslaco, in the Lower Rio Grande Valley, from 1924 to 1930, are summarized in this report. It is realized that experimentation over a much longer period will be required to settle definitely many of the points raised in the present report. This publication serves a double purpose: it gives an evaluation of the work in progress, and also makes available to the Valley citrus grower such tentative conclusions as the results warrant up to the present. The citrus industry in the Lower Rio Grande Valley, as shown in Table 1, although of recent growth, takes a high rank among the horticultural industries of the State. Nationally, the Valley ranks among the three leading citrus-producing areas; in grape- fruit production, it ranks second only to Florida. From an output of approximately 15 carloads of grapefruit in 1921 the citrus industry of the Valley has expanded to a produc- tion of more than 4,000 carloads in 1929-30. The data given in Table 1 are conservative, since shipments by express and truck are not included. The rapid increase shown in the table gives an accurate indication of the trend. The total number of citrus trees planted in the Lower Rio Grande Valley up to July 1, 1929, as shown in Table 2, numbered 5,118,981; of these 72 per cent were grapefruit, 13 per cent of which were 5 years of age or older (37) i‘ While the greater part of the Texas citrus acreage of bearing age is located in the Lower Rio Grande Valley, plantings have been made in the Laredo and Winter Garden districts and the Upper Gulf Coast region (20). The present report is concerned only with the industry in the Lower Rio Grande Valley. Although experiments in citrus fruit production have been conducted in other producing areas, it does not follow that results obtained elsewhere are directly applicable to conditions in the Lower Rio Grande Valley. Soil and climatic factors differ greatly from those found in California, Florida, and Arizona (39, 40, 42, 7, 31, 41). It is therefore necessary to con- duct fundamental experiments under the conditions found in the Lower Rio Grande Valley in order to determine the most profitable practices to be followed in growing citrus fruit in this region. *Chief Division of Horticulture, 1928-1930; Horticulturist, U. S. Depart- ment of Agriculture since July 1, 1930. TNumbers in parentheses refer to literature citations, p. 58. 6 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION Table 1. Car-lot shipments of citrus fruits from the Lower Rio Grande Valley by i‘ Shipments by express and truck not included 1921-22 to 1929-30, inclusiveT Car lot shipments Year Railroad _ _ Totals on . Grapefruit Oranges Lemons Mixed each G ¢ citrus railway, to _ 1921-22. . . Mo. Pac. 7 1 0 3 . . . . . . . . .. 1 . 1922-23. . . Mo. Pac. 44 0 0 7 . . . . . . . . . . 5 1923-24. . . Mo. Pac. 107 0 1 7 . . . . . . . . . . 11 1924-25. . . Mo. Pac. 50s 1 1 11 . . . . . . . . . . 52 1925-26. . . Mo. Pac. _ 290 1 0 0 . . . . . . . . . . ' y, 1926-27. . . Mo. Pac. 706 11 0 39 . . . . . . . . . . 7 -‘ 1927-28. . . Mo. Pac. 903 27 0 86 1016 .-, So. Pac.* 140* 11 v_‘_ 1928-29. . . Mo. Pac. 1311 28 0 112 1451 , So. Pac.* 299* 17 *5- 1929-30. . . Mo. Pac. 2898 114 0 365 3377 -- ' So. Pac.* 604* 398 ’ TData furnished by Missouri Pacific and Southern Pacific Railways. *Reported as total carlots not classified. Table 2. Citrus planting in the Lower Rio Grande Valley of Texas as of July 1, 1 1- Totals for Cameron, Hidalgo,’ and Willacy counties (37) Number of growing citrus trees of different ages i Class Under One Two Three Four Five To one year year years years years years »~ and over Grapefruit . . . . . ..1,319,103 916,334 458,232 297,084 224,662 487,3343,722, Oranges. . . . . . . . .. 367,236 280,298 181 ,100 157,434 138,802 195,7441,320, Other citrus . . . . . . 13,485 7,638 ' 5,717 8,923 10,580 29,275 75, Total . . . . . . . 1 ,699,824 1 ,204,270 645,049 463,441 394,044 712,353 5,118, i’ Soils- The soils on which citrus fruits ‘are grown in the Lo i Rio Grande Valley vary considerably as to their physical chemical properties, ranging from the rather light sandy lo ¢_ soils of the Brennan and Victoria series to the heavier clay 10a 1; of the Rio Grande and Laredo series. It is generally conce‘ that the well-drained, deep sandy loam soils such as Victo Brennan, and Hidalgo fine sandy loams, are best suited to cit fruit production (4, 10, 11, 14, 15). However, some excelle orchards are found growing on Victoria clay loam where natu y drainage is adequate. Most of the soils of the Valley which used for crop production compare very favorably, as regar fertility, with the principal types used for crop production y other parts of the state (10). i “ Climate- The climatic conditions in the Lower Rio Gran Valley are in general favorable for crop production. The : CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS -7 nual rainfall amounts to approximately 23 inches, the greater portion of which falls during the periodsfrom May to June, in- clusive, and from September to November, inclusive (21, 38). Because distribution is not satisfactory it is usually necessary to supplement the rainfall with irrigation water. The growing season is relatively long in this region, extend- ing from February to December, and temperature favorable for the growth and development of citrus trees is the usual condi- tion during this period. From April 1 to November 1, the monthly mean temperature ranges above 70 degrees. Critically 10W temperatures are occasionally experienced during the months of December, January, February, and March. The prevailing direction of the wind is from the southeast, and the moisture-laden air from the Gulf usually maintains the humidity at about 75 per cent, which is favorable for plant growth (38). Scope 0f Publicativn- Since the various classes oflcitrus fruits are rather closely related genetically and the cultural require- ~ ments are similar, the experimental results concerning some factors in citrus-fruit production in the various classes are treated in a single publication. The subject matter is con- veniently grouped under the following heads,—(a) Citrus Va- riety Standardization, (b) Citrus Root-stocks, and (c) Grape- fruit Orchard Management. CITRUS VARIETY STANDARDIZATION Probably the most important benefit to be derived from standardization of citrus varieties is the elimination of various inferior forms which make it difficult to maintain a constant supply of a relatively few varieties of special merit which can be grown in sufficient quantities, and which the consumer will recognize and demand. An added advantage is that standard- ization will simplify the problems of both the grower and the nurseryman since it will enable them to specialize on the pro- duction of a relatively few forms. In this connection the needs of the industry must be given first consideration. Varieties should be introduced or developed which mature their fruit relatively early (prior to December 15), in order to avoid pos- sible loss due to low temperature. Since consumers have de- cided preferences as to the size of fruit which they purchase, this character should receive due consideration. Also, it should be indicated that the factors responsible for quality in citrus fruits are probably of more importance than is generally recog- nized. The proportion of rind and “rag,” the number of seeds per fruit, and the quality of the juice in terms of solids to acids ratio, or a still better measure if it can be found, must be con- 8 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION sidered. The capacity of the fruit to hold up well in transit A and storage is also of primary importance. The experience of growers during the past has shown that up to the present time the grapefruit is more profitable than other classes of citrus in the Valley. This greater relative importance is apparently due to the wider adaptability range of grapefruit as compared with other classes of citrus fruits. (See Table 3.) This fact, however, does not preclude the possibility of the in- troduction or development of forms in other classes of citrus which will be equally well adapted. The data presented in this section under citrus variety standardization are concerned with (a) the systematic study of the plant as a whole as it responds to the environmental condi- tions of the Valley, and (b) the factors which affect quality and other standards in citrus fruits. Plan of Standardization Experiments The standardization experiments, located on‘ Victoria fine sandy loam soil, may be conveniently grouped into ecological studies and quality studies. . Ecological Studies- The method followed in determining the sum total of environmental factors as affecting citrus plants consisted in growing, whenever possible, 3 or more trees of each item studied under the usual orchard practices in the Valley. The trees were planted in orchard form and the following records were taken: “ripening season,” degree of frost resistance, keep- ing quality, and a general adaptability rating. These terms are explained in detail under “Explanation of Terms.” Yield rec- ords are not included in the present report, since most of the trees are relatively young. Quality Stlldies- In these studies it was the aim to study the quality of citrus fruits under the conditions of normal orchard practices as now followed in the Valley. The physical characters of the entire fruit and the quality of citrus juice were subjected to detailed analysis. The following determinations were made in the case of the entire fruit: total weight in grams and the proportion of “rag,” juice, and seeds; thickness of rind, diameter of pulp in millimeters, and thenumber of seeds. The quality of the juice was studied on the basis of total soluble solids, kind of sugars, total acids, effective acidity (pH), Van Slyke buifer in- dex, protein, and ash. The methods of procedure have been published elsewhere (34, 35, 36). The terms used are defined under “Explanation of Terms.” Sampling- The constants for fruit character are based upon random samples of commercial grade fruit. Whenever-possible, ten or more fruits were utilized as a sample. CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 9 Explanation of Terms The experimental data have been summarized in Tables 3, 4, and 5. The statistical analysis of the data given in Tables 4 and _5 are presented elsewhere (34, 35, 36). The facts concerning adaptability of cultivated forms of citrus from various sources, grown under Valley conditions, from 1924 to 1930, are shown in Table 3. The cultivated forms have been grouped according to the classification by Swingle (2, 16). The following definitions of special terms apply to the data presented in Tables 3 and 4. “Ripening season” refers to the period when the major por- tion of the crop reaches maturity and may be harvested; “Early” refers to the period between October 15 and December 1; “Mid- season,” December 1 to January/l; and “Late,” January 1 to March 1. The term “Frost Resistance” is used as a measure of the capacity of the plant to withstand temperatures below 28 degrees F. The sign “—|-,” is used to designate varieties commonly un- injured at 28 degrees F.; “++,” refers to varieties that are uninjured at 23 degrees F.; “—” designates forms which are injured at 28 degrees F. _ “Keeping quality” is a broad term used to distinguish rather conspicuous differences in perishability as observed by packers and shippers. “Adaptability rating” is a general term used to indicate the effect of the sum total of environmental factors as found in the Lower Rio Grande Valley on citrus plants. Three degrees of adaptability are recognized: “good,”—vigorous and prolific forms; “fair,”—medium vigorous and prolific forms; and “poor,” —forms not vigorous, unproductive or unable to survive. “Rag” is that portion of the fruit remaining after the juice has been extracted by means of a conical citrus-fruit extractor, and after the rind and seeds have been removed. The physico-chemical characters of citrus juice presented in Table 5 were determined as follows: total soluble solids, by means of a Brix spindle at room temperature, correcting for temperature variations and expressed at 22 degrees C. ; sugars, protein, and ash, by direct analysis; total acids, by titration with 1/10 normal alkali solution, and expressed as anhydrous citric acid; pH or effective acidity, on an electric hydrogen ion apparatus to three decimal places; buffer index (Van Slyke), based on pH determination after adding 1 equivalent of acid to 1000 cc. of juice. 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N. 32> JOEE mwwom 532m waning. .§N.o.o .22. oioaamoma ..§~.¢ dmfiom "wish .638 "$34 swam .355 x$§io 53ft. éii v28 éwcmfimsow won kwwfiébmmm.; dug 2am .$m>_m=<.. CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS amigxm $5 R: .1. .1. .1. .1. 8.3 2: Nfifi .1. QQNQN Fo:.wQ HUZ(Q.\¢.§ X ‘>\<\.\$\\o\ %\Q\§\\Qm\§ WXUQ\Q~ \Q \§%§\m\.m\%\\b \Qm\§ w\vkm\b\ Ebb m\.\bm\\ QQQQW. \\\\\.\1 \B§b\\m W.\\wQ\m\Qb WkisoQm, Exs QukQ \D\Qm kbkmmmflwko Qb\n\ .z T?‘ \\ m Uh ‘Nvmvxwk T d CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 37 Rio Grande Valley as described above both previous to and during the period covered by this Work, excepting where experimental treatments required a variation in this routine. Critically low temperatures were experienced in December, 1925, when the temperature dropped to 25 degrees F. The prin- cipal injury to the trees was in the nature of breakage caused by ice formation on the limbs and leaves. Only slight defoliation followed this cold period and most of the trees recovered in approximately two years. Critically low temperatures Were ex- perienced also during 1929-30, when the temperature again dropped to 25 degrees in December and to 22 degrees F. in Jan- uary. The fruit had been removed from the trees at the time of the January freeze and yields were, therefore, unaffected for this season. Only slight bark injury was noted on the 11-year- old trees and defoliation was so slight as to be negligible. Scale activity during the season of 1926-27 caused consider- able injury to some trees in the orchard. The gum diseases be- came a factor of primary importance during the season of 1926 following the 1925 freeze. By using the recommended surgical treatment for this disease and then painting the wound with a solution of denatured alcohol, bichloride of mercury, and rosin (12), practically all of the affected trees recovered within a period of two years. Normality 0f Yields- In considering the data presented in Tables 11 and 12, it should also be realized that out of the five years, only three have been normal as to yield, primarily on account of unusual seasonal influences in two seasons. This shows the necessity of caution in interpreting the results. This indicates that more weight should possibly be given to results obtained in normal years than to a five-year mean, for instance. The history of the orchard would indicate that the season 1929-30 is probably the most typical of the behavior to be expected under the condition of experiment as to yields secured to date. It has been pointed out that during the season 1925-26 the trees were unduly affected by low temperatures during December, which caused marked injury to some trees on account of break- age from ice accumulation on foliage, limbs, and fruit; the following seasons were fairly representative of what one could expect from trees recovering from the injury of 1925-26. Dur- ing the fourth year, 1928-29, the ravagesjof scale insects led to ‘partial defoliation of a considerable number of trees. In 1929- 30, no undue seasonal influences were encountered before the crop was harvested. Yields from Abnormal Trees- The yields from abnormal trees were discarded for the purpose of this experiment on the fol- lowing basis: yields of broken, diseased, and insect-damaged 38 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION trees, Where such damage obviously affected yields, Were omitted; annual yields were also discarded if the trees Were unduly affected by sporadic attacks of red scale. Yield Records» As a measure of performance, the total yield per tree Was taken to the tenth pound. It became apparent later that such a measure was too refined, and the yield data_ l have therefore been uniformly expressed as 100-lb. boxes per tree, which is the logical measure for grapefruit. The smallest unit used is a tenth of such a 100-lb. box, and is therefore equal to 10 pounds. Table 8. l Blank experiment with grapefruit, entire orchard, 1924-25 Before differential treatments were begun V Mean Mean Mean Mean yield yield yield . yield Plat per tree* Plat N0. per tree* Plat No. per tree* Plat N0. per tree* 100-lb. 100-lb. 100-lb. 100~lb. boxes boxes boxes boxes A~1.. 0.3 B-1..... 0.9 C- 0.3 D- 0.8 2.. 0.6 0.8 0.4 0.7 3.. 0.4 1.2 0.5 0.6 4.. 0.3 1.0 0.9 0.7 5.. 0.4 1.2 1.3 0.9 6.. 0.4 1.0 1.3 0.8 7.. 0.6 1.3 1.3 0.8 8.. 0.5 1.7 1.3 0.5 9.. 0.6 1.6 1.3 0.8 10.. 0.6 10..... 1.2 10..... 1.1 10..... 0.7 11.. 0.7 11..... 1.1 11..... 1.2 11..... 0.7 12.. 0.7 12..... 1.4 12..... 1.4 12..... 0.9 13..... 1.7 13..... 1.1 14..... 1.6 14..... 0.8 15..... 1.6 l5..... 1.0 16..... 0,9 16..... 1.0 17..... 1.5 17..... 1.0 18..... 0.9 18..... 1.2 19..... 1.5 19..... 1.6 20..... 1.0 20..... 1.3 21..... 1.7 2l..... 1.2 22..... 1.7 22..... 1.0 23..... 1.0 23..... 0.8 24..... 1.6 24..... 0.8 25..... 1.2 25..... 1.1 26..... 1.5 26..... 0.8 27..... 1.6 27..... 1.1 28..... 1.8 28..... 1.0 29..... 1.3 29..... 0.9 30..... 1.5 30..... 0.9 31..... 2.0 31..... 0.7 32..... 1.5 32..... 0.7 33..... 2.0 33..... 0.8 34...... 2.1 34..... 1.6 35..... 1.5 35..... 1.2 36..... 1.7 36..... 0.6 37..... 1.4 37..... 0.7 38..... 1.2 38..... 1.0 39..... 1.4 39..... 1.4 40..... 1.0 40.. 0.8 *Yield on 5 tree plat basis, and expressed as mean per tree. CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 39 Table 9. Blank experiment with grapefruit, 1334-25; continuity plats C-13 to 29, an d I)-13 to Mean yield per tree,* expressed in 100-lb. boxes Fdat P¢o.* 1924-25 1925-26T 1926-27 1927-28 1928-291 1929-30 (113 . . . . . . . . . . . . . . .. 1.67 1.45 1.91 2.28 1.01 4.38 14 . . . . . . . . . . . . . . .. 1.63 1.48 1.46 2.09 1.01 4.06 15 . . . . . . . . . . . . . . .. 1.65 . . . . . . . . .. 1.83 2.41 1.16 3.65 16 . . . . . . . . . . . . . . .. 0.88 . . . . . . . . .. 1.45 2.67 1.29 4.26 17 . . . . . . . . . . . . . . .. 1.53 . . . . . . . . .. 1.53 3.29 1.78 4.71 18 . . . . . . . . . . . . . . .. 0.92 . . . . . . . . .. 2.04 3.31 2.35 4.66 19 . . . . . . . . . . . . . . .. 1.48 . . . . . . . . .. 2.63 3.54 2.72 5.49 20 . . . . . . . . . . . . . . .. 1.02 . . . . . . . . .. 1.91 2.91 1.47 4.34 21 . . . . . . . . . . . . . . .. 1.68 . . . . . . . . .. 1.60 2.83 1.76 5.79 22f . . . . . . . . . . . . . .. 1.71 . . . . . . . . .. 1.08 3.27 2.60 5.58 23 . . . . . . . . . . . . . . .. 1.00 . . . . . . . . .. 1.05 3.11 2.56 4.91 24 . . . . . . . . . . . . . . .. 1.60 . . . . . . . . .. 1.10 4.09 1.92 6.39 25 . . . . . . . . . . . . . . .. 1.24 . . . . . . . . .. 2.06 4.09 2.94 5.79 26 . . . . . . . . . . . . . . .. 1.55 0.54 0.81 3.40 1.38 5.37 27 . . . . . . . . . . . . . . .. 1.63 0.45 1.37 3.71 1.70 6.43 28 . . . . . . . . . . . . . . .. 1.82 0.68 1.75 4.36 2.53 5.32 (129 . . . . . . . . . . . . . . .. 1.33 0.84 2.21 4.54 2.50 5.78 I)13 . . . . . . . . . . . . . . .. 1.08 1.49 1.94 2.84 2.13 4.49 14 . . . . . . . . . . . . . . .. 0.78 . . . . . . . . .. 1.85 1.96 2.03 3.74 15 . . . . . . . . . . . . . . .. 0.99 . . . . . . . . .. 1.92 2.46 2.38 4.63 16 . . . . . . . . . . . . . . .. 1.01 . . . . . . . . .. 2.34 2.45 2.28 5.40 17 . . . . . . . . . . . . . . .. 0.96 . . . . . . . . .. 1.27 2.88 2.04 4.81 18 . . . . . . . . . . . . . . .. 1.18 . . . . . . . . .. 1.37 3.03 1.42 5.13 19 . . . . . . . . . . . . . . .. 1.59‘ . . . . . . . . .. 2.45 3.11 1.59 6.46 20 . . . . . . . . . . . . . . .. 1.27 . . . . . . . . .. 2.26 2.69 1.82 3.22 21 . . . . . . . . . . . . . . .. 1.22 . . . . . . . . .. 1.84 2.45 2.10 5.73 22 . . . . . . . . . . . . . . .. 0.97 . . . . . . . . .. 0.90 2.27 1.78 5.07 23 . . . . . . . . . . . . . . .. 0.76 . . . . . . . . .. 0.82 2.42 2.07 5.81 24 . . . . . . . . . . . . . . .. 0.80 . . . . . . . . .. 2.49 3.26 1.57 4.52 25 . . . . . . . . . . . . . . .. 1.14 0.59 0.50 ’3.45 0.88 5.60 26 . . . . . . . . . . . . . . .. 0.84 0.40 1.39 3.13 1.28 5.40 27 . . . . . . . . . . . . . . .. 1.15 0.82 2.48 3.89 1.32 6.68 28 . . . . . . . . . . . . . . .. 0.98 0.49 1.19 3.17 1.37 5.29 {X29 . . . . . . . . . . . . . . .. 0.88 0.78 1.29 3.77 2.12 6.60 *Yields on 5-tree plat basis, and expressed as mean per tre_e. TCertain plats omitted due to damage irom freezing of fruit. IYields lowered by severe scale infestation. EXperimental Error- In carrying ~ on experiments in orchard management, it is necessary to give due consideration to experi- mental error, Which makes difficult the accurate interpretation of the results secured under any particular conditions of soil, climate, plant material, plant pests, and cultural practices (13, 8, 27, 23, 24, 25). The scientific method requires that all other conditions be held constant while one or a combination of two or more are varied. The practical orchardist knows only too well that this is not strictly possible under orchard conditions where such factors as bud-mutation* (26, 23, 24, 25) in trees, soil fer- tility, damage from plant pests, and low temperature may not affect all the trees uniformly. However, when the danger from these sources is fully realized, it is possible by the application of proper statistical methods to determine the approximate relative performance under the experimental conditions together with *Possibly not of much importance from the standpoint of total yield. 40 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION some estimate of the accuracy 0f such approximations. Such values Will serve for practical purposes. Plat Variability- In order to secure a rational basis for inter- preting the data secured it is desirable to determine the amount of variation over the entire orchard. Johnston (17) in 1849, from a theoretical viewpoint, emphasized the importance of de- termining “the limits of variation in natural productivity of the field,” i. e., the (comparative yields of all the plats of the experi- mental field Without differential treatment, as a necessary step in field experimentation, It Was not until the 90’s, however, that extensive blank field experiments Were carried out by Larsen (18). Anthony ( 1) in 1927 used the principle of performance records in apple fertilizer experiments, and Batchelor, Parker, and McBride (3) in 1928 reported on a blank experiment with citrus trees prior to their use in a nutrition experiment. In order to secure an index of the amount of variation over the entire orchard, the grapefruit crop Was harvested Without differential treatments for the season 1924-25. These yields are shown in Table 8. As a continuous measure of variation for the duration of the experiment, the yields from a portion of the orchard, hereafter called “continuity plats” (Plats C-13 to 29, Table 10. Analysis of blank experiments; plat variability as influenced by size of plats lVIean _ Number yield Coeffi- Size of plat, feet of trees Number Season per tree Standard cient of per plat of plats 100-lb. deviation variability boxes I. Entire orchard, 1924-25 21 x 21 . . . . . . . . . . .. 1 550 1924-25 1.11.01 0.58 52.7 21 x 105 . . . . . . . . . . .. 5 104 1.11.02 0.37 33.6 21 x210 . . . . . . . . . . .. 10 52 1.11.02 0.26 23.6 II. Continuity plats (C-13 to 29 and D-13-29), 1925-30 21 x 21 . . . . . . . . . . .. 1 70 1925-26 0.81.03 0.47 58.7 21x 105 . . . . . . . . . . .. 5 12 ' 0.81.06 0.36 46.0 21 x 210 . . . . . . . . . . .. 10 5 * ’ * * 21 x 21 . . . . . . . . . . .. 1 155 1926-27 1.51.06 1.12 74.6 21 x105 . . . . . . . . . . .. 5 34 1.51.06 0.59 39.3 21 x 210 . . . . . . . . . . .. 10 18 1.51.07 0.46 30.6 21 x 21 . . . . . . . . . . .. 1 169 1927-28 3.11.05 0.99 31.6 21 x 105 . . . . . . . . . . .. 5 34 3.11.07 0.69 22.2 21 x 210 . . . . . . . . . . .. 10 18 3.11.10 0.65 20.9 21 x 21 . . . . . . . . . . .. 1 168 1928-29 1.81.04 0.91 50.5 21 x 105 . . . . . . . . . . .. 5 34 1.81.05 0.53 29.4 21 x 210 . . . . . . . . . . .. 1O 18 1.81.06 0.41 22.7 21 x 21 . . . . . . . . . . .. 1 160 1929-30 5.11.08 1.44 28.0 21 x 105 . . . . . . . . . . .. 5 32 5.11.10 0.83 16.2 21 x210 . . . . . . . . . . .. 10 16 5.11.11 0.66 . 12.8 *Omitted on account of small number of plats. CITRUS PRODUCTION IN THE LOWER. RIO GRANDE VALLEY OF TEXAS 41 and D-13 to 29), were harvested each year Without differential treatments. These yields, from 1924-25 to 1929-30, are shown in Table 9. A general idea of the amount of variation over the parts of the orchard included in these blank experiments may be secured by inspecting the data in Tables 8 and 9. However, a more accurate and quantitative measure of the variability may be secured by calculating the coefficient of variability (C. V.), which indicates the amount of variation over the entire area considered in terms of percentage of the average yield per plat. In Table 10, the coefficient of variability has been Worked out for the blank experiments on the basis of 1-tree, 5-tree, and 10- tree plats. In general, when the size of plats is increased without increas- ing the total area, there is a decrease in the coefficient of variability. When the yield data for the entire orchard are expressed in terms of 1-tree plats, the variation ranges from 28 to 74 per cent of the mean or average plat yield over the period of 5 years. This extreme variation throughout the entire orchard is reduced to a range of 16 to 46 per cent when the same data are expressed as 5-tree plats. Expressing the data on a 10-tree plat basis gives in general a further reduction in variabil- ity, but the amount of reduction is not as consistently large as it is when 1-tree and 5-tree plats are compared. The relatively large number of trees per plat required for experimental purposes to overcome the indicated variability would make orchard experiments of this kind prohibitive if the ordinary method were to be used of contrasting treatments with check plats distributed over the entire orchard (1, 19, 30, 33). An economical method of statistical analysis in harmony with the facts must therefore be adopted. Methods of statistical analysis should of course be used only for valid biological reasons. It is imperative, on this account, to consider carefully not only the type of plant material studied under the particular conditions of growth as already indicated but also the stage or stages in the development of the plant organism in which the quantitative measurements (variates) were secured. The pomologist, with few’ exceptions, deals with perennial woody p1ants,—trees and shrubs. Such plant material is often studied in the developmental stage,—the grand period of growth, during which yields are subject to progressive increases over a period of years. From the standpoint of statistical analysis, when the grand period of growth is ended and the stage of so-called maturity is reached in trees, the conditions are probably somewhat comparable to those which obtain in the case of annual crops. When the stage of decline or senescence sets in the conditions are again altered. In the present case, the grapefruit trees were 5 years old when 42 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION the experiment was begun, and have developed over a 5-year l- period. The absolute yields have, in general, reached a higher level with increasing age of the trees. ' Since we are not dealing with an absolute yield level, it is erroneous to consider a fictitious mean yield for the period on the basis of the normal curve of dis- tribution where fluctuations about a mean value are logically con- sidered as due to errors of random sampling. Unless each season is considered separately, it is clear that the use of probability tables, based upon the normal curve of distribution, are not jus- tified. It has been shown, however, that variation in plat yields over the entire orchard is too great to make feasible the interpre-- tation of data for single seasons on the basis of the comparison of widely separated plats. The making of seasonal paired com- parisons of'adjacent plats on the basis of the consistency of differences would make it possible to escape from the difiiculty presented in the case of plants studied as developing organisms. Method ef Interpreting Data- It follows from the preceding dis- cussion of plat variability that even if adjacent orchard plats are given the same cultural treatments for the duration of the experiment they will vary to some degree one from the other. However, as a general rule, plats adjacent or near each other will have a tendency to vary less one from the other than plats distributed in distant parts of the orchard for environmental factors, such as soil fertility, drainage, etc., will tend to be sim- ilar for both. By considering plats adjacent or near each other some of the variable factors affecting the trees are eliminated (22). Since 1908, there has been available a method, commonly known as “Student’s” Method (28, 29, 30), which meets the re- quirements of our problem: (a) it makes possible the use of paired comparisons, and (b) it is applicable to small numbers. By this method groups of contrasting pairs may be compared on the basis of the consistency of individual gains in estimating the significance of the average difference. When this method is employed, in the present instance, the yields of 5-tree plats of grapefruit trees adjacent or near each other and receiving dif- ferent treatments may be compared, and the results interpreted on the basis of the consistency of the gain of one plat over the- other. The making of paired comparisons was not original with “Student,” and his real contribution to methods of statistical analysis is due to the development of probability tables which are applicable to small numbers,—-2 to 30 variates. When large numbers are available it is possible to calculate quite accurately" the value of the standard deviation of a mean value. In such a case the calculated results for the purpose of determining the CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 43 significance of a difierence Would naturally be referred to probability tables based upon the normal curve of distribution. When relatively small numbers are the only data available these tables Would not give reliable indications of the significance of a difference since We have, then only an estimate of the true standard deviation. In the development of “Student’s” tables (28, 29, 30, 19) this fact Was taken into consideration. It is not claimed that results from small samples are as reliable as those based upon large numbers of variates. It is true, how- ever, that the use of “Student’s” tables eliminates the mathe- matical error that Would enter in if probability tables based on the normal curve Were used When mean values and their standard deviations from the means have been calculated from relatively small samples. The Worker in such a field of economic botany as horticulture must assume a practical attitude, and must ex- pect to encounter the condition of small populations When dealing With plant materials such as grapefruit trees which are not as cheaply produced as annual crops. So long as the due caution is exercised in the interpretation of the results under the condi- tions there is little likelihood of going astray. It has been pointed out above that probability tables have been developed by “Student” (29, 19) to Which the calculated results, according to “Student’s” formula, from any group of contrasting pairs may be referred for the purpose of securing an indication of the significance of the results. The probability tables give the odds that such differences as may be obtained are due to a cause or causes Which affected one of the pairs and not the other and is not the result of chance variation. When the experiment is so conducted that the factors which influence one side of the pairs and not the other is reduced to the minimum, except the differential treatment required by the experiment, then the odds indicated give a measure of the sig- nificance of the increases secured from the differential treatment. It is the usual practice to conclude that a certain treatment is better than another, when the odds are 30 to 1 or higher that a given difference is the result of differential treatment and not of chance variation. When the odds are less than 30 to 1, further proof is required before We may conclude that the difference obtained is due to the differential treatment and not to chance. This condition applies especially When one is dealing With small numbers. Utilization 0f Plats- Out of a total of 104 plats, containing 5 trees each, 20 Were devoted to studies in cultivation, cover crops, and mulches; 48 Were used for fertilizer Work; and 38 Were reserved for a continuous blank experiment as indicated in the preceding discussion. 44 BULLETIN NO. 419, TEXAS AGRlCULlURAL EXPERIMENT STATION I CULTIVATION, COVER CROPS, AND MULCHES Experiments concerning cultivation, cover cropping, and mulching in citrus orchards have been reported by workers in California (39, 40) and Florida (31, 41). The results in gen- eral show the necessity of maintaining the organic content of the soil at a productive maximum. This is borne out especially by the Rubidoux experiments with citrus in California (39, 40) , and for crops in general by the classical experiments at the Rotham- stead Experiment Station in England. There is considerable variation in the season of the year when cover crops are chiefly grown in the important citrus-producing regions. In Florida cover crops are grown during the rainy season, May to October; in California they are produced from December to March, and i.n Texas, summer cover cropping, from May to August, is the general rule. . The present experiments Will serve as a first step in determin- ing, on an experimental basis, the most economical methods of cultivation, cover cropping or mulching under Valley conditions. Plan of Experiment The experimental work concerning cultivation, cover cropping, and mulching as factors in maintaining the Valley grapefruit orchard at maximum bearing capacity was planned to include ten contrasting treatments: (a) Modified clean culture- This system of cultivation and cover cropping consists of disking under the weeds that appear after each irrigation. Whenever possible, weeds are not allowed to grow to a height of more than two feet before they are disked under. No effort is made to keep the ground entirely free of weed growth. This is the method commonly followed by many Valley citrus growers on account of the fact that it becomes difiicult to keep Valley citrus orchards free of weeds during certain periods of the year. (b) Modified clean culture with 6-inch plowing. When this system is followed, a strip about 8 to 10 feet wide between the trees is plowed to a depth of 6 inches and then the method described under “modified clean culture” is followed. ((3) Winter cover crops,-—non-legume. This methgd Consists of growing a crop of oats or barley on the soil during the season from November to April and then incorporating it with the soil. Subsequent management is the same as that described under “modified clean culture.” i (d) Winter cover crvpr-legume- Crops of yellow annual Sweet Clover, Melilotus indica, are grown during the period from ' 'm.1...; CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 45 November to March and then incorporated with the soil by disk- ing. After this, “modified clean culture” is given. (e) Summer cover crops,-—legume. CrQpg 0f cgwpeag are grown during the period from May to August, inclusive; followed by “modified clean culture.” (f) Intermittent s0d—legume and culture. Alfalfa and sweet clover were allowed to grow undisturbed for a period of three years and were then incorporated into the soil. “Modified clean culture” was followed the fourth year. (g) Continuous sodrloguino- The land is kept seeded to alfalfa for an indefinite period. No cultural tillage is given. (h) Mulohod basin- Natural grass and weeds are allowed to grow and are cut down with scythes several times each season and used to mulch around trees. No cultural tillage is given. (i) Continuous sod,—culture about trees. Natural grass and weeds are allowed to grow undisturbed except that a small area around the trees is kept hoed free of vegetation. No other cultural tillage is given. Continuous sod,—no culture about trees. Natural grass and weeds are allowed to grow undisturbed. No cultural tillage is given. ' Utilization of Plats- A total of twenty 5-tree plats were avail- able for this work, which made it possible to replicate each of the above ten treatments twice. Practical limitations dependent on available irrigation laterals made it necessary to arrange the treatments in two parallel series, Plats C-3O to 39, and D-30 to 39; C-29 and C-40, D-29 and D-40 serving as border plats. The plat arrangement is shown in Figure 1. The treatments are indicated in Table 11. Duration of EXporiInoni» The experiment was conducted for a period of 4 years, 1925-26 to 1928-29. The work had to be dis- continued after the season 1928-29 on account of the fact that by this time the branches of the 8-year-old trees touched in the middle of the rows. This condition made it impractical to grow“ cover crops as originally planned. Interpretation of Results The results have been analyzed by the application of “Stu- dent’s” Method on the basis of comparing adjacent plats. This does not make it possible to compare “Modified Clean Culture,” for instance, directly with other treatments not adjacent. How- ever, the comparative value of any particular plat may be deter- mined by comparison with adjacent plats on both sides, and these in turn may be compared with plats farther removed. 46 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION mco$m6~m> vucmso 6o ism»! w mm won 6cm 666E635 o5 6o 25mm! N mm m6?» 6:05.66 c626 QZN: m6cuawmoh~ iwficuhowm6 036 o5 6N5 606x365 Gm mm 69666.56 95 .5663 .5 Q o» 6m mm 66E mm m66O doing?» 6on9? o6 6:6 Ho: m6 Amxownuv 33a 6wfi$bqs .8>o 636N666 6o m6_2> E 6066.666 o5 6N5 m66O** , 263E QQNQm oZmmvoxo an comwom mFS 63:63 v53 m6QoCWw dkswmponaog 32 we 666x986 .3551 .6356: 2m 33m 695A 6on3 63:03 3m mismfi $6.3m $626 06.56 so 63mm? . 656336 >6 6.6 m; 6.6 6m Q muub 655E 52$ 53mm 66656605 mEQ. fim 66+ >6 m6 6A 6.6 6m U 3516 on .6om Q3625 msosqficoU , 660E633 m.6 hm 6.6 6.6 mm Q moob 650mm miS 5:3 32mm 6.666326 Q6“. m6| 66 mm mN m.6 mm U 3315 .6om 625m: mnoscflcoU 6666395 mm 6.6 “N 6.6 hm Q 5N5 .6366 UEvEfiNEQ mEQ. QJ 56+ mN Nb. 6m m6 mm U . . . . . . . . . . . . . . . . . . imam 604232 66666095 mm N6 m; m6 6m Q E5 68:5 66656665 mEQ. in 56+ 5N v.6 5m 6.6 6m U . . . . . . . . . 65:63 .6om msoaafiaoU 666E633 m6 mm 6.6 v.6 mm Q . $516 min 52$ $362 miwmazm 1: N6| 9N 6.6 6.m m6 mm U 6cm uEzwoQ 60m Emfimizzfi 66581.6»: N N mm 9m i. m». Q 6 m5... 5:3 6630a 656M526 Q6 Toll mm m6 mm 6 .6 6m U . . . . . . . 65:62 60.5 695v 66:65am @5385 mN he MN :6 mm Q can» 6666mm Hcucbwwfi mEQ. finm 6.6+ m6 6.6 mm n6 mm U .. . . . . . .5566“: .603 $>oo 63st,? wi6wuoha 6A hm mN 6.6 Nm Q E23 5:2 66656.65 mEQ. fim mm6+ Q .6 6.6 6.6 66 N». U . 1.6566250: .6060 $>oo .5653 655:6: m; m...“ NA :6 E Q mnezzg 25 5:3 $3.3 $68.56 HQ m66| 6A 6.6 m; m6 5 U 6056 .3516 6x26 606M602 6A 6.m 6A >6 6m Q 6.6 v.6 6A 6.6 6m U ............o;::o E3? 606M602 mwxon . .562 52a mwxon mwxon moxon moxon 6666336 .562 .562 A662 .562 **m66O $>o mmNwQ M66662 wm-nmi hm6m6Q $N-mN2 dZ 62m 65656365 .5 :6 260E 5T6 “womb. $6 662m n32 666mg .393 #:6606666. 6o 6666» no $6265 6cm macho .8>oo iofiafifiuo 6o 0060565 .: BANE CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 47 It will be noted, by referring to Table 11, that 0n the basis of a 4-year-average loss of 0.03 box, or 3 pounds, per tree, the odds are 1:1 that “Modified Clean Culture with 6-inch Plowing” has caused a lesser yield than “Modified Clean Culture.” Since odds of 30 :1 are considered on the border line of significance, we con- clude that there is no significant difference, and that nothing has been gained or lost by the additional 6-inch plowing. Similarly, the 4-year-average gain of 0.2 box per tree in favor of “Winter Cover Crop,-—Non-legume” as compared with “Modi- fied Clean Culture with 6-inch Plowing” shows odds of only 2:1 that this gain is due to the treatment and not t0 chance varia- tion. Clearly the gain is not significant. When, however, “Winter Cover Crop,—Legume” is contrasted with “Winter Cover Crop,—Non-legume,” the 4-year-average gain of 0.4 box per tree in favor of the first treatment is shown to be significant. The odds are 87:1 that this treatment has caused consistent gains over a 4-year period. The Al-year-average differences, gains or losses as the case may be, between “Summer Cover Crop,—legume” and “Winter Cover Crop,—legume,” between “Intermittent sod—legume and culture” and “Summer Cover Crop,—legume,” between “Continuous sod, —legume” and “Intermittent sod—legume and culture,” and be- tween “Mulched Basin” and “Continuous sod,—legume” are ap- parently not significant, since on the basis of comparison of adjacent plats the odds that these differences were due to the treatments received and not to chance range from 1:1 to 11:1. We conclude therefore that these five types of treatment have given about the same responses as far as yields are concerned during the 4 years covered by the experiment. Further experi- ments are necessary over a longer period of years to determine the relative importance of the various treatments which have given the most favorable responses so far. When the “Mulched basin” system is compared with “C0ntin- uous natural sod,-—culture about trees,” there is an apparent annual average loss of 0.8 box per tree as a result of the latter treatment. The odds are 70:1, and therefore significant that this system of sod culture was responsible for the loss. No significant difference was revealed between the two methods of sod culture: the one with culture about trees, and the other without culture about trees. The odds are only 2:1 that the latter is better than the former. Application of Results The four years’ results indicate that better yield responses, a gain of approximately 0.4 box per tree, were obtained in grapefruit culture by methods of seasonal or continuous cover cropping with legumes, or a system of mulch- ing, than were secured by the commonly used method of “Modi- 48 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION fied clean culture,” or systems of continuous sod culture. Al- l though the results are quite conclusive as regards the two groups of cultural practices, the evidence is not conclusive with refer- ‘ ence to the relative importance of the treatments which have ¢ given most favorable increases in yield. This may be due to f the fact (a) that the experiment Was conducted on relatively ; fertile virgin soil and covered a period of only 4 years, and (b) that the trees Were only 9 years old at the end of the experi- § ment. It is possible that the natural fertility of the soil made A it impossible to reveal differences great enough to be measured under the conditions. With mature trees (10 to 20 years of age) over a similar or longer period of years, the relative values of these outstanding treatments may be obtained. For the present, it Will be safe to recommend a system of seasonal leguminous cover cropping with tillage rather than the customary method of clean cultivation with only sporadic natural cover crops. Deeper plowing, 6 inches once per season, does not seem to be of practical value, when added to the system of “Modified clean culture.” FERTILIZER APPLICATION It is natural that Where large crops are removed from the land annually, even in the case of virginally fertile soils, the plant food Will ultimately become exhausted and must be re- placed. The question of economical fertilizer application must therefore be considered sooner or later. Consequently, in other citrus-producing areas fertilizer experiments have been carried on for some time. Work carried on in California (39, 40) over a period of year points to the fact that nitrogen is a critical ele- ment in citrus-fruit production on the soils utilized. Similar experiments conducted in Florida (7, 31, 41) seem to indicate the advisability of using complete fertilizers. Plan of Experiment The present experiment concerning grapefruit fertilization was undertaken With the object of determining (a) the time required for exhausting the natural fertility of the soil to such a point, by cropping With grapefruit, Where the application of commercial fertilizers will become profitable, and (b) the grade and amount of fertilizer mixture required to keep productivity at the most profitable point When the time arrives that fertilizer applications are profitable. Plats Available- Exclusive of border plats, a total of forty 5- tree plats Were available for these experiments: A-2 to 11; B-2 to 11; C-2 to 11; and D-2 to 11. The location of the plats with CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 49 reference to the entire experimental orchard is shown in Fig- ure 1. Kind 0f Treatments- A total of 18 different treatments are in- cluded in the experiment. The fertilizer formula, 4-8-4 (4 per cent nitrogen; 8 per cent phosphoric acid; and 4 per cent potash) , was adopted as the standard grade or formula for the treatments With inorganic fertilizers. Treatments were arranged to test the three fertilizer elements, nitrogen, phosphoric acid, and pot- ash (a) in combination as a so-called complete fertilizer (N-P-K) ; (b) in pairs (two fertilizer elements, O-P-K N-O-K, and N-P-O) ; and (c) alone (one fertilizer element, N-O-O, O-P-O, and O-O-K) . In addition to these treatments, phosphoric acid was used in one-half (4-4-4), and potash in double (4-8-8) amounts with full amounts of the other two as required by the standard formula. In one treatment, consisting of nitrogen alone, one-half of the amount was applied in the spring, and the other half in the summer. In one case cottonseed meal was added to the standard treatment (4-8-4) with inorganic chem- icals. The following sources of the fertilizer elements were used: (a) nitrogen,—nitrate of soda; (b) phosphoric acid,- superphosphate; and (c) potash,—sulphate of potash, and muriate of potash. Organic fertilizers (barnyard manure, bone meal, and cotton- seed meal), hydrated lime, gypsum, flowers of sulphur, and iron sulphate were applied alone. The amounts and kind of fertilizers constituting the various treatments are conveniently indicated in the first column of Table 12. Replication- The relatively large number of treatments, 18 in all, precluded the possibility of much replication. As 11 plats were reserved as checks receiving no treatment, two replications were possible with 11 treatments; this left 7 treatments with- out replicates. The relatively small number of replications will necessitate carrying on the experiment over a long period of years to make up in part for this deficiency. r Application of Fertilizers» The various treatments have been in- dicated in actual weight of fertilizer material applied. In or- der to reduce the possibility of cross feeding to a minimum, the greater part of the amount was applied to the soil between trees in the same row, but small amounts were also applied on the other two sides. No fertilizer was placed on the soil compris- ing the irrigation borders between plats. 50 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION Em N..N m.N m.N m.6 . . . . . . . CERES 6N-U Nnm N6+ m6 N.m N..N N.m >6 . . . . . . . . . . . . . . ..NN|U 6.6 N.m m.N m.N N..6 . . . @886 N. E8 NfiQ Qafimmag Eva m5 m 1mm N\6+ 6.m» m.m N.N m.m N.N . . . . . . . Im-Q 2.3? @332 d5mNN N we m.m m.N m.m N..6 ..Nwv_8.§ NN E8 NnQ NNN N6l. N..m m.N» m.N Nim E6 .. . . . . . . . . . . 13.6mm . N 6 N .N m.m m.N m6 . . . . .. . . . .0565; v-0 593cm mo 8235 .5 6N\m NHN N6] m.m m.N 6.m m.N m6 wNVowmQoNwSEdQNmNNN . ma“ mum mmN m6 N..6 125358 NN E8 NnO N6v m6|| N.m mm 6 N mN E6 . . . . . . . . . . . . . ....m-Q m.m N .N N.m m.N E6 . . gfioonov N. E8 v-0 538m mo BEE=w .5 6N\m NnN Ni6+ Nim m.m N.m m.m N.6 .. . . . . . . . . .......m-U Bmnmwamg Eow a5m 3:085» mvwmzfiom 9,3. .8 uommmozmm< .NN . N.m E6 N..N m.N m6 ...... . . 8.25s 61m 58E Nvowmcofioo d5 N~ NUNNN No.1 N..m 66 m.N EN m.6 ................NN-mN mNmmNommoufiwmmmsw .56N\m N535 ENE N N + m.N 6.6 m.6 . . 8x026: 6 E8 NW4 vmwnmwonm Eon d5 m %H§ + $.$ .-.. . . . . .........@1< N@QW%QUPNHP:M x N535 urn-E N.N + m.N 6.6 m.6 . . 1350856 6 E8 NW4 523cm .6 2231a .m5 2S N NJ“ m6+ m.m + m.N E6 m6 . . . . . . . . ...:m-< Bfiwmmfim Eva .w5 m 28w mo @5256 d5 mNN N . 6.N m.N mmN m.N m6 . . 282E 6N 6mm NnU 593cm mo @2235 .5 6N\m NUNN m 6+ N.m N.N m N m.m m6 . . . . . . . . . . . 16-0 vmmamwonm Eva d5 NNN N 38m mo @625: a5 mNN N N585 v3-3 6N + 6. N N. N m6 . . . . . . . . .QNuoNNov 6-4 593cm No 063825 .5 6N\m NUN N.6+ 6 m + N.N m.N N6 ................6N-< $521222 Eon a5 m 3.8 mo 3x5? a5 mN N N . we m.m m.N m.m N..6 .. $50053 NN E5 NnnN NUN N 6+ 6.m m.m m.N m.m E6 . . . . . . 267G £89m? Swing .5 6N\m . Nmm NNN m.m m.N m6 .........§uv58 NHU oNasmmomm 66w .95 m #ux m» % @ € Am.» %.§ @.m @.% .-.-. . . . . . ......§IU NrvOW%QUPNHHNNH § mmofimfiom omommfioo No #6335154 .N 3b 8m @082 38a 38a $85 385 M325 .5-66N .5-66N .5-66N .5-66N .5|66N .5|66N 3.5 8m .8213“ $25.0 .5835. .688 6m|6N6N NmNlwNmN wNlNNmN N.N|mN6N +mN|mN6N doZ NEAN r38 afifitfi mo NEE E8 E5054 wwE 8 8E0 53E Emvm vwoob 8m N583 NNMQE 6m|mN6N .395 mmsmmommmm mo E2» no wmvummmhom mo oomosmcN .NN 255. 51 CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS acompmwzw> wocmao 0o £53 m mm “o: 0cm ficozfiaofi o5 0o :53 a mm mEvw» 02000000 A630 9E: wgonfimuhw iwflauhowmw 9,3 23 02S coSwodAvE cm mm UPAQUEAAOO 3w H.505 acofimmbg momma“. o0 ~50 0cm 2 3x253 $20 wofiwPfics .550 wvwmvb 0o 0E3 E Qucmlwwmw Q5 $33 @000: 5.5.30 vAmum 0cm 3:0 ufimwooxw 3 0:0 030050 32ml dbfimhoafifi B2 0n wwwooba 2:275. .0323: PE 2E0 35A :2?» A6253 3m 339E 0005mm "$20 v0.5-0 0o Ewan 00* .5 A 3 00 mm @010 mm @000 0m + 0.A mA + . . . . . . . $28000 0-< A0 90+ 0.0 + Aim v.0 h. . . . . . . . . . . . . . . . 30-4 $52033 :9: .9: fiA . . 0.m + 0.A m.A 0.0 . . . . . . . icsfiov 0-4. . A.0 m 0+ 90 + 0.m 0.0 v.0 .. . . . . . . . . . . . . . ..v|< .5001; 0o E0300 m5 $A . . 0.0 0.0 9m 9A v.0 Amfiwasmwfi 90A . .€.N .1 mw.@ @@. . . . . . . . . .........%Im wgw 0.0 v.0 9m 9A v.0 $00003 00cm 90A . . . - . . . . » . . . - . . . . . . . . . . . . . . 203E056 $50 UAAm 0E: 0o co$wuaam< > . 0.0 0.0 9m 9A v.0 A.0 A.0+ 0.0 0.0 0.m 0.A v.0 . . . . . . . . . . . . . . . Iv-m 9A + A.A 0.0 v.0 . . . . . . . . 10225 w)» . AAA 0.0+ v.A + AiA A.A 0.0 . . . . . . . . . ..\ . . . . 10% 13E waissoo 3A Xv . 0.0 0.A 0.m 0.A 0.0 Afioopoviza Wm A.0 A.0+ 0.0 0A 9m 0A 90 . . . . . . . . . . . . . . . IAWMA . . v.0 0.0 v.m 9A 0.0 . . . . . . . . 30.00230 0-4 . A.v v 01! 0.0 + 0.A m.A 0.A . . . . . . . . . . . . . . .AA-< 03E 95A Q: 0A . . v.0 “v.0 v.m 9A 0.0 . . . . . . . . .2025 00A A.m A 0|| 0.0 0A Aim AiA 0.0 . . . . . . . . . . . 2070A . . 9A + A.A 0.0 v.0 ..........Av_n.2s¢-< . A.0 0 0+ 0.0 + AA A.A 90 . . . . . . . . . . . . ..m-< ohscmi 03.3w 3000A» $32350 259.05 wnoim> 0o uomfiwozna< .>A . . 0.0 9A 0.m 0.A 0.0 Afioflzovmwam m-m A.A A 0+ 0.0 0.A A.m v.A 0.0 . . . . . . . . . . . . . . . 200A . . 0.0 0.m 9m Aim 0.0 :........2§§ AHO A.0 A~ 0+ 0.0 0.0 9m m4“ 0.0 . . . . . . . . . . . . . . . 19D 093cm 0o msfiizdm .5010 . . A.0 A.m 90 0.m 0.0 .:.:..:EQQ§WQ Anm A0+ 0.0 A.0 m.0 A.0 0.0 0.0 A.m m0 9m 0.0 Afiowfiv 2:5 TU . A00 v.0+ 90 0m m.0 0.0 v.0 . . . . . . . . . . . . . . . ..0-U Bwiwona 06m d5 0 0.0 0.A 0.m 2 90 .0080“; 0A AEw v-U .€um<~ mw.@ @.© . . . . . . . . . . . . . - . ..%|U N@Ow %o QPQHHAAH .WQ@ § A.0 A.m 90 0m 0.0 C0508 Afifl .5885 AEm AA A.0| 90 0A 0.0 v.m 0A . . . . . . . . . . . . . 305A wciaw 2.3 0o 035;: 0.33.03 m 313:3» .5§:._._vA 0:9 AD ZDSsQA-mmfi 4: 52 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION Method of Interpreting Results It has been emphasized in the discussion of cultivation and cover cropping that in an experiment with relatively young trees (4 to 10 years old) grown on fertile virgin soil, it may be pos- sible to show only the marked differences over such a compara- tively short period of 5 years. The experiment must be con- tinued long enough to include also the behavior of maturegrape- fruit trees (10 years or older). The analysis of the 5-year data, however, will shed some light on the degree of natural fertility of the soil, which is one of the purposes for conducting the experiment. The second enquiry regarding the grade and amount of fertilizers to apply will become of importance only When nutrient deficiencies become apparent. The interpreta- tion of the results at the end of 5 years, in the case of young trees which were 4 years old at the beginning of the experiment and are now 10 years old, under the conditions of the experiment must be approached with due respect for these facts. Yields without Fertilizer Application. Seasonal variations in yields are quite marked, as would be expected in the case of develop- ing trees. The trees, four years of age, at the beginning of the continuity experiment as shown in Tables 9 and 10, averaged one 100-pound box of fruit per tree. During the following years seasonal mean yields per tree were: 1925-26, 0.8; 1926-27, 1.5; 1927-28, 3.1; 1928-29, 1.8; and 1929-30, 5.1 boxes. These yields were secured without the use of fertilizer. It will be noted that when the trees in this experiment had attained the age of 10 years the per tree yield was comparable to that secured from well-cared for commercial orchards of similar, age, approxi- mately 4.0 100-pound boxes. Use 0f “Studenifs Method- The results have been interpreted according to “Student’s” method, the contrasted pairs consisting of a treated plat and a mean or average value arrived at by con- sidering the nearest check plats on both sides of the treated plat. Where this procedure was not possible, the nearest check plat alone was utilized as the other member of the pair. The complete results are presented in Table 12. Application of Complete Fertilizers. Ag ghgwn in Table 12, a Com- plete fertilizer composed of 2.6 pounds of nitrate of soda, 5 pounds of acid phosphate, and 0.8 pound of sulphate of potash gave indicated increases of 0.6 and 0.1 box per tree over a 5- year period. On the basis of the consistency of these increases, the odds, according to “Student’s” tables, range from 1 :1 to 8:1 that these indicated differences are consistent and therefore sig- nificant. In the light of the results from the continuity experi- ment, where no fertilizer was applied, and the yields are appar- CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 53 ently normal as judged by comparison with yields from com- mercial orchards 0f the same age, such results as secured by the application of a complete fertilizer would seem to indicate that soil fertility has not as yet become a limiting factor under the con- ditions of the experiment. In the case of all the remaining treatments with complete fertilizers as shown in Table 12, similar results have been se- cured so far. The odds range from 1 :1 to 14 :1 that such treat- ments have caused increases or losses as the case may be, and are therefore, not significant. Application of Two Fertilizer Elements. When two fertilizer ele- ments are applied, as shown in Table 12, the indicated gains or losses due to the treatments, over a 5-year period, are apparently not significant. Two exceptions appear. A loss of 0.6 box per tree in the case of a treatment with 5 pounds of acid phosphate and 0.8 pound of sulphate of potash with odds of 40:1 is counter- balanced by an indicated gain of 0.4 box in another similar treatment with odds of 1:1 in another part of the orchard. Similar contradictory results were secured for the treatment of two plats with 2%- pounds of nitrate of soda and 5 pounds of acid phosphate. Application of One Fertilizer Element. Ag would be expected frem the results discussed thus far, the gains or losses indicated as associated with the treatment with one fertilizer element are apparent rather than real. The odds range from 1:1 to 3:1 that the difierences indicated are due to the treatments, except- ing in one instance. When 5 pounds of acid phosphate was applied in one case the indicated gain was 0.7 box, with odds of 35:1, but another identical treatment in another part of the orchard gave an indicated increase of 0.1 box per tree with odds of only 2 :1 that the difference is due to the treatment and not to chance variation. Organic Fertilizers, Lime and Other Chemicals. When different plats were treated at the rate of 400 pounds of stable manure, 10 pounds of bone meal, 75- pounds of cottonseed meal, 12 pounds of hydrated lime, 12 pounds of gypsum, 1% pounds of flowers of sulphur, and 1.1; pounds of iron sulphate per tree in eachlcase, the oddsgthat increases or decreases indicated are significant range from 3:1 to 20:1. In all except one case, the odds are 11:1 or below that the differences are due to the treatments and not to chance variation. When hydrated lime was applied at the rate of 12 pounds per tree an indicated mean anuual in- crease of 0.5 box per tree is shown, and the odds are 20:1 that this difference is significant, but in the absence of a replicate, 54 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION the results must be observed over a longer period before any I reliance can be placed on the odds approaching 30:1. Discussion of Results- The results secured over a five-year period with fertilizer treatments seem to show that on the basis of the application of “Student’s” method, increases or decreases indi- cated are not significant. Several explanations are possible: (a) soil fertility may not as yet be a limiting factor in this orchard; (b) variation in yields due to differences in soil, plant materials, etc., may be important; (c) the differences caused by the treatments may not be great enough to be revealed by the ‘technic of analysis used; or (d) cross feeding may occur. In view of the fact that consistent results Were secured by the application of “Student’s” method in the interpretation of data secured with varying treatments in the cultivation, cover cropping, and mulching experiment, it would appear that the method of analysis used is adequate, and that cross feeding has not been an important factor up to the present. Although there was a rather great variation in yields over the entire orchard at the beginning of the experiment as shown in Table 8, the yields of the continuity experiment, shown in Tables 9 and 10, indicate that this variation is becoming generally less and less as the orchard reaches maturity. The elimination of yields from abnormal trees, and the comparison of plats which are adjacent or in close proximity also has the tendency to re- duce plat variation from such causes as differences in soil fer- tility, injury from pests, etc., to the minimum. It appears, therefore, that the inconsistent results from the application of fertilizers under the conditions of the experiment, up to the present, are due to the favorable virginal fertility of ‘the soil. That the fertilizer elements applied under the experi- ‘mental conditions were apparently not limiting factors up to the present time is borne out by the yields indicated in the con- tinuity experiment as pointed out above. Although these trees received no fertilizer applications, their development and yields appear normal. Application 0f Results The results from the fertilizer experi- ments thus far seem to indicate that the Valley citrus grower is favorably situated as regards natural soil fertility. These re- sults are in essential harmony with the practice of not utilizing fertilizers until the trees reach bearing age. The results, however, should not be interpreted as meaning that fertilizers should be withheld after the trees reach bearing age since moderate applications along with other orchard prac- tices throughout the development and maintenance of the orchard "will tend to keep the original fertility of the soil unimpaired. Figure 2. Spacing of grapefruit trees. Upper—10-year Marsh trees spaced 21x21 ft., Weslaco Station; lower—10-year Marsh trees spaced 30x30 ft., Donna, Texas. ‘56 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION SPACING GRAPEFRUIT TREES In laying out the grapefruit orchard one should allow sufficient space between the trees to facilitate such necessary orchard practices as cultivation, cover cropping, orchard heating, pest control, fruit harvesting, and the maintenance of irrigation borders. Planting distances will depend in the main upon the growth habits of the variety and root-stock, the fertility of the soil, and the amount of irrigation water available, or the amount -of rainfall. Although grapefruit trees planted relatively closer together are better protected from wind and frost, close spacing has the effect of crowding out the lower fruiting branches. The spacing distances formerly used in the Valley, 21x21 feet, as used in California (42), are apparently not suited to local conditions of high soil fertility and favorable climatic factors for growth. As a general rule grapefruit trees grow vigorously in the Lower Rio Grande Valley, and after 8 years the branches -of adjacent trees spaced 21x21 feet, usually touch. This inter- feres seriously with cultivation and other orchard practices, and as has been pointed out, the lower branches of the trees are unduly shaded. Proper spacing distances for grapefruit trees in the Valley seem to be about 25x25 feet. As shown in Figure 2, trees of the Marsh variety in the Station orchard on Victoria fine sandy loam soil, spaced 21x21 feet, are crowded at the end of 10 years. Trees in a similar orchard of the Marsh variety at Donna, Texas, on the same type of soil, spaced 30x30 feet, do not show crowding at the end of 10 years. However, this wide spacing will tend to reduce acre yield during the first six or eight years of bearing. ACKNOWLEDGMENTS The experiments dealing with the various factors that enter into citrus production in the Lower Rio Grande Valley, including standardization of varieties, citrus root-stocks, fertilizer appli- cation, use of tillage, cover crops and mulches, etc., were initiated by W. H. Friend and A. T. Potts in 1924. Experiments on quality in citrus were initiated by H. P. Traub and W. H. Friend in 1928. Thanks are due Dr. G. S. Fraps for advice in planning the fertilizer experiments and for assistance in connection with the analytical work on citrus quality. CONCLUSIONS 1.1 The annual citrus-fruit shipments from the Lower Rio Grande Valley of Texas reached approximately 4000 carloads by 1929-30. 2. More than 5,000,000 citrus trees were growing in orchard CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 57 form in the Lower Rio Grande Valley as of July 1, 1929; of these, approximately 75 per cent Were grapefruit, 13 per cent of which were 5 years of age or older. 3. On the basis of adaptability t0 local conditions, physical character of the Whole fruit, and quality of juice, the relative merits of various types of citrus fruits have been studied during the period from 1924 to 1930. 4. The Marsh grapefruit and Thompson, a pink-fleshed bud- mutation of Marsh, are recommended for general planting in the Valley. These varieties are desirable because of their rela- tive “seedlessness” and superior quality of juice. 5. The desired tartness in sweet oranges as grown under Valley conditions seems to be present in late varieties like» Valencia; early varieties like ParsonBrown are apparently lack- ing in this respect. Of the early varieties, Hamlin is recom- mended for general planting on account of its relative “seedless- ness” and excellent quality. However, this variety lacks the qualities desirable in a good “shipping” orange. Of the late varieties, Valencia is recommended as the standard. This xrariety possesses excellent “shipping” quality and the desired tartness of juice. 6. The Clementine or Algerian tangerine is recommended as the most desirable variety in this group, because of its early maturity and superior quality. 7. Ordinary commercial forms of lemons are too tender to frost to be profitable in this region. From the standpoint of general adaptability, especially frost resistance, a special form, the Meyer lemon, takes first rank in this group of citrus fruits. 8. The commercial forms of limes are too tender to frost to be profitable in this region. A special form, Rangpur lime, al- though quite hardy to cold, is lacking in quality. 9. The kumquat is one of the hardiest forms of citrus grown in the Valley and is well adapted to local conditions. 10. The following citrus root-stocks are recommended for the various" types: (a) grapefruit, sweet orange, tangerine, and lime on sour orange; (b) Satsuma orange on Rusk Citrange; (c) Meyer lemon on its own roots; (d) Kumquat on rough lemon; or Rusk Citrange. 11. Four years’ results with cultivation, cover crops, and mulches in grapefruit culture indicate that beftter yield re- sponses, an annual gain of approximately 0.4 box per tree, were obtained by the use of leguminous cover crops and by a system of mulching than were secured by the commonly used method of ' “modified clean culture,” or non-leguminous cover cropping. 12. Five years’ results with differential fertilizer treatments. in grapefruit culture indicate that the materials and amounts. used have not produced significant increases in yield. 58 BULLETIN NO. 419, TEXAS AGRICULTURAL EXPERIMENT STATION 13. A spacing distance of 25x25 feet is apparently more desirable for grapefruit in the Lower Rio Grande Valley than a closer spacing. The 21x21-foot spacing used -in the early days of the industry is too close for this region. LITERATURE CITED (1) Anthony, R. D. orchard experiments by the use of “Student’s Method.” Amer. Soc. Hort. Sci. 23: 71-73. (2) Bailey, L. H. 1924. Macmillan, N. Y. (3) Batchelor L. D., E. R. Parker and Robert McBride. 1927. Planning and analyzing apple Proc. Manual of cultivated plants. 1928. Studies preliminary to the establishment of a series of fertilizer trials in a bearing citrus grove. Calif. Agr. Expt. Sta. Bul. 451. (4) Beck, M. W., and B. H. Hendrickson. 1927. Soil sur- vey of Cameron County, Texas. U. S. Bur. Soils Bul. (5) Bonn, W. W., and Mertz, W. M. 1916. Experiments with stocks for citrus. Calif. Agr. Expt. Sta. Bul. 267. (6) California State Department of Agriculture. 1927. The California fruit, nut, and vegetable standardization act of 1927 . As amended, 1929. Calif. State Printing Office, Sac- ramento. (7) Collison, S. E. 1919. Florida Agr. Expt. Sta. Bul. 154. (8) Collison, R. C., and Harlan, J. D. 1930. Variability and size relations in apple trees. N. Y. (Geneva) Agr. Expt. Sta. Tech. Bul. 164. (9) Florida State Department. cof May 29, 1929. (10) Fraps, G. S. 1913. The composition of the soils _of iSouth Texas. Texas Agr. Expt. Sta. Bul. 161. Pp. ~110-114. (11) Fraps, G. S., and S. E. Asbury. 1928. Commercial “fertilizers in 1927-28 and their uses. Texas Agr. Expt. Sta. Bul. 387. ' (12) Friend, W. H. 1928. Report of Supt. Substa. 15, Weslaco. In 40th Annual Rept. Texas Agr. Expt. Sta. (13) Harris, J. Arthur. 1928. Further studies on the per- ‘formance of differences in the plats of an experimental field. Jour. Agr. Res. 36: 15-40. (14) Hawker, H. W., and M. W. Beck. 1929. A soil sur- vey of Hidalgo County, Texas. U. S. Bureau of Chemistry and Soils Bul. 13. (15) Hawker, H. W., and C. S. Simmons. 1930. A soil survey of Willacy County, Texas. U. S. Bureau of Chemistry and Soils Bul. 3. Citrus fertilizer experiments. 1929. Citrus maturity law CITRUS PRODUCTION IN THE LOWER RIO GRANDE VALLEY OF TEXAS 59 (16) Hume, H. H. 1929. Cultivated citrus fruits. Mac- millan, N. Y. (17) Johnston, J . F. W. 1849. Experimental. agriculture. Edinburgh and London. (18) Larsen, B. 1897. Om metoder for baltforsok. Andra Nordiska Landtrukskongressen i Stockholm. 1: 72-84. (19) Love, H. H. 1924. A modification of “Student’s” table for use in interpreting experimental results. J our. Am. Soc. Agron. 16: 68-73. (20) Potts, A. T. 1912. Report on experiments with citrus fruits at the Beeville Substation. Tex. Agr. Expt. Sta. Bul. 148. (21) Potts, A. T. 1924. The Lower Rio Grande Valley of Texas.- Tex. Agr. Expt. Sta. Circ. 34. (22) Sax, Karl. 1924. Fertilization of apple orchards in Maine. Me. Agr. Expt. Sta. Bul. 322. (23) Sax, Karl. 1928. Bud and root selection in the Apple. Me. Agr. Expt. Sta. Bul. 344. 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